ssr_primer
Primer Details for TRINITY_DN70806_c19_g1_i2 |
|---|
| (A)14: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACATCGCATAGCCCATCGTT | TCTCGAATGTTCATACGGCGT | Start: 122 |
| Tm | 59.893 | 59.866 | End: 226 |
| Size | 20 | 21 | 105 |
| Primer 2 details |
|---|
| Sequence | GACATCGCATAGCCCATCGT | TCTCGAATGTTCATACGGCGT | Start: 121 |
| Tm | 60.32 | 59.866 | End: 226 |
| Size | 20 | 21 | 106 |
| Primer 3 details |
|---|
| Sequence | ACGACATCGCATAGCCCATC | TCTCGAATGTTCATACGGCGT | Start: 119 |
| Tm | 60.32 | 59.866 | End: 226 |
| Size | 20 | 21 | 108 |
| Primer 4 details |
|---|
| Sequence | ACGACATCGCATAGCCCATC | TGTTCATACGGCGTTCTTCCT | Start: 119 |
| Tm | 60.32 | 59.726 | End: 219 |
| Size | 20 | 21 | 101 |
| Primer 5 details |
|---|
| Sequence | ACATCGCATAGCCCATCGTT | CTCGAATGTTCATACGGCGT | Start: 122 |
| Tm | 59.893 | 58.443 | End: 225 |
| Size | 20 | 20 | 104 |
(T)23atgaggaaaattgtgaata(T)10aaattttcagccgtcttatatcaaattat(A)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACATCGCATAGCCCATCGTT | TCTCGAATGTTCATACGGCGT | Start: 122 |
| Tm | 59.893 | 59.866 | End: 226 |
| Size | 20 | 21 | 105 |
| Primer 2 details |
|---|
| Sequence | GACATCGCATAGCCCATCGT | TCTCGAATGTTCATACGGCGT | Start: 121 |
| Tm | 60.32 | 59.866 | End: 226 |
| Size | 20 | 21 | 106 |
| Primer 3 details |
|---|
| Sequence | ACGACATCGCATAGCCCATC | TCTCGAATGTTCATACGGCGT | Start: 119 |
| Tm | 60.32 | 59.866 | End: 226 |
| Size | 20 | 21 | 108 |
| Primer 4 details |
|---|
| Sequence | ACGACATCGCATAGCCCATC | TGTTCATACGGCGTTCTTCCT | Start: 119 |
| Tm | 60.32 | 59.726 | End: 219 |
| Size | 20 | 21 | 101 |
| Primer 5 details |
|---|
| Sequence | ACATCGCATAGCCCATCGTT | CTCGAATGTTCATACGGCGT | Start: 122 |
| Tm | 59.893 | 58.443 | End: 225 |
| Size | 20 | 20 | 104 |
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