ssr_primer
Primer Details for TRINITY_DN70786_c4_g1_i1 |
|---|
| (T)15: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ATGTTGCTGTGCTGAAAGGC | TTCTTTACTGGGCTGCCAGG | Start: 184 |
| Tm | 59.684 | 59.962 | End: 357 |
| Size | 20 | 20 | 174 |
| Primer 2 details |
|---|
| Sequence | GGGGCTTGCAGACACAAAAA | CGGCGGTTTCTAAGTTGTGC | Start: 315 |
| Tm | 59.536 | 60.11 | End: 519 |
| Size | 20 | 20 | 205 |
| Primer 3 details |
|---|
| Sequence | TACTTTGGGGCTTGCAGACA | CGGCGGTTTCTAAGTTGTGC | Start: 309 |
| Tm | 59.524 | 60.11 | End: 519 |
| Size | 20 | 20 | 211 |
| Primer 4 details |
|---|
| Sequence | ATGTTGCTGTGCTGAAAGGC | TTTTTGTGTCTGCAAGCCCC | Start: 184 |
| Tm | 59.684 | 59.536 | End: 334 |
| Size | 20 | 20 | 151 |
| Primer 5 details |
|---|
| Sequence | TGTTGCTGTGCTGAAAGGCT | TTCTTTACTGGGCTGCCAGG | Start: 185 |
| Tm | 60.753 | 59.962 | End: 357 |
| Size | 20 | 20 | 173 |
(A)10gaaaaaaaatacctaaagaatagtaataactaaattgt(A)10ttatctttctt(A)11: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ATGTTGCTGTGCTGAAAGGC | TTCTTTACTGGGCTGCCAGG | Start: 184 |
| Tm | 59.684 | 59.962 | End: 357 |
| Size | 20 | 20 | 174 |
| Primer 2 details |
|---|
| Sequence | GGGGCTTGCAGACACAAAAA | CGGCGGTTTCTAAGTTGTGC | Start: 315 |
| Tm | 59.536 | 60.11 | End: 519 |
| Size | 20 | 20 | 205 |
| Primer 3 details |
|---|
| Sequence | TACTTTGGGGCTTGCAGACA | CGGCGGTTTCTAAGTTGTGC | Start: 309 |
| Tm | 59.524 | 60.11 | End: 519 |
| Size | 20 | 20 | 211 |
| Primer 4 details |
|---|
| Sequence | ATGTTGCTGTGCTGAAAGGC | TTTTTGTGTCTGCAAGCCCC | Start: 184 |
| Tm | 59.684 | 59.536 | End: 334 |
| Size | 20 | 20 | 151 |
| Primer 5 details |
|---|
| Sequence | TGTTGCTGTGCTGAAAGGCT | TTCTTTACTGGGCTGCCAGG | Start: 185 |
| Tm | 60.753 | 59.962 | End: 357 |
| Size | 20 | 20 | 173 |
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