Primer Details for TRINITY_DN70766_c2_g2_i1 | |||
|---|---|---|---|
| (A)23: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGCCTGTCTTCTTTATCACGG | TGTGTAGAAGCACATAAGGTTCAC | Start: 103 |
| Tm | 59.073 | 59.005 | End: 239 |
| Size | 21 | 24 | 137 |
| Primer 2 details | |||
| Sequence | GCCTGTCTTCTTTATCACGGGA | TGTGTAGAAGCACATAAGGTTCAC | Start: 104 |
| Tm | 60.094 | 59.005 | End: 239 |
| Size | 22 | 24 | 136 |
| Primer 3 details | |||
| Sequence | CACGGGAGGATGTAAAATGTGC | TGTGTAGAAGCACATAAGGTTCAC | Start: 119 |
| Tm | 59.903 | 59.005 | End: 239 |
| Size | 22 | 24 | 121 |
| Primer 4 details | |||
| Sequence | CGCCTGTCTTCTTTATCACGG | TGTGTAGAAGCACATAAGGTTCACT | Start: 103 |
| Tm | 59.073 | 60.222 | End: 239 |
| Size | 21 | 25 | 137 |
| Primer 5 details | |||
| Sequence | CGCCTGTCTTCTTTATCACGG | TGTGTAGAAGCACATAAGGTTCA | Start: 103 |
| Tm | 59.073 | 57.778 | End: 239 |
| Size | 21 | 23 | 137 |
