ssr_primer
Primer Details for TRINITY_DN70701_c2_g3_i3 |
|---|
| (T)19: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACCACCAATGACGGTCTTCC | ACCCCGGAATCCCAAATTCC | Start: 1546 |
| Tm | 59.964 | 60.033 | End: 1650 |
| Size | 20 | 20 | 105 |
| Primer 2 details |
|---|
| Sequence | ACCACCAATGACGGTCTTCC | CCCGGAATCCCAAATTCCCA | Start: 1546 |
| Tm | 59.964 | 60.033 | End: 1648 |
| Size | 20 | 20 | 103 |
| Primer 3 details |
|---|
| Sequence | AACTACTGGGCAGACGAAGC | GTTGAGACGCCTGACTGACA | Start: 2438 |
| Tm | 60.037 | 59.968 | End: 2577 |
| Size | 20 | 20 | 140 |
| Primer 4 details |
|---|
| Sequence | GTCGTACCCTGGCAAAGACA | GGGGGCAGAGGACAATGAAA | Start: 3425 |
| Tm | 59.966 | 59.96 | End: 3525 |
| Size | 20 | 20 | 101 |
| Primer 5 details |
|---|
| Sequence | GTCACCGCCTCCTGAAATCA | AGCCGCATTTTGAGCACTTG | Start: 1924 |
| Tm | 60.036 | 60.039 | End: 2191 |
| Size | 20 | 20 | 268 |
(T)14: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACCACCAATGACGGTCTTCC | ACCCCGGAATCCCAAATTCC | Start: 1546 |
| Tm | 59.964 | 60.033 | End: 1650 |
| Size | 20 | 20 | 105 |
| Primer 2 details |
|---|
| Sequence | ACCACCAATGACGGTCTTCC | CCCGGAATCCCAAATTCCCA | Start: 1546 |
| Tm | 59.964 | 60.033 | End: 1648 |
| Size | 20 | 20 | 103 |
| Primer 3 details |
|---|
| Sequence | AACTACTGGGCAGACGAAGC | GTTGAGACGCCTGACTGACA | Start: 2438 |
| Tm | 60.037 | 59.968 | End: 2577 |
| Size | 20 | 20 | 140 |
| Primer 4 details |
|---|
| Sequence | GTCGTACCCTGGCAAAGACA | GGGGGCAGAGGACAATGAAA | Start: 3425 |
| Tm | 59.966 | 59.96 | End: 3525 |
| Size | 20 | 20 | 101 |
| Primer 5 details |
|---|
| Sequence | GTCACCGCCTCCTGAAATCA | AGCCGCATTTTGAGCACTTG | Start: 1924 |
| Tm | 60.036 | 60.039 | End: 2191 |
| Size | 20 | 20 | 268 |
(T)10cattgtcctctg(C)14: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACCACCAATGACGGTCTTCC | ACCCCGGAATCCCAAATTCC | Start: 1546 |
| Tm | 59.964 | 60.033 | End: 1650 |
| Size | 20 | 20 | 105 |
| Primer 2 details |
|---|
| Sequence | ACCACCAATGACGGTCTTCC | CCCGGAATCCCAAATTCCCA | Start: 1546 |
| Tm | 59.964 | 60.033 | End: 1648 |
| Size | 20 | 20 | 103 |
| Primer 3 details |
|---|
| Sequence | AACTACTGGGCAGACGAAGC | GTTGAGACGCCTGACTGACA | Start: 2438 |
| Tm | 60.037 | 59.968 | End: 2577 |
| Size | 20 | 20 | 140 |
| Primer 4 details |
|---|
| Sequence | GTCGTACCCTGGCAAAGACA | GGGGGCAGAGGACAATGAAA | Start: 3425 |
| Tm | 59.966 | 59.96 | End: 3525 |
| Size | 20 | 20 | 101 |
| Primer 5 details |
|---|
| Sequence | GTCACCGCCTCCTGAAATCA | AGCCGCATTTTGAGCACTTG | Start: 1924 |
| Tm | 60.036 | 60.039 | End: 2191 |
| Size | 20 | 20 | 268 |
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