ssr_primer
Primer Details for TRINITY_DN70649_c3_g6_i1 |
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| (T)27: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTGTTGAAACCATGCACCGG | CTGAGGTGGCTTTGGTTCCT | Start: 642 |
| Tm | 59.613 | 59.889 | End: 764 |
| Size | 20 | 20 | 123 |
| Primer 2 details |
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| Sequence | TTCCAAAATGACACCCGCCA | CTGAGGTGGCTTTGGTTCCT | Start: 492 |
| Tm | 60.468 | 59.889 | End: 764 |
| Size | 20 | 20 | 273 |
| Primer 3 details |
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| Sequence | CCCGCCAGTTCCGTTAAAGT | CTGAGGTGGCTTTGGTTCCT | Start: 505 |
| Tm | 60.605 | 59.889 | End: 764 |
| Size | 20 | 20 | 260 |
| Primer 4 details |
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| Sequence | ACCCGCCAGTTCCGTTAAAG | CTGAGGTGGCTTTGGTTCCT | Start: 504 |
| Tm | 60.605 | 59.889 | End: 764 |
| Size | 20 | 20 | 261 |
| Primer 5 details |
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| Sequence | TTCCAAAATGACACCCGCCA | TTTCATGGACACCGGCATGT | Start: 492 |
| Tm | 60.468 | 60.251 | End: 690 |
| Size | 20 | 20 | 199 |
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