ssr_primer
Primer Details for TRINITY_DN70624_c0_g1_i3 |
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| (A)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGTGCGGCACTTCATTTGTG | AGAAAGCTGAGTCATGGGCC | Start: 2187 |
| Tm | 59.969 | 60.035 | End: 2405 |
| Size | 20 | 20 | 219 |
| Primer 2 details |
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| Sequence | AGGACTGGTGGCACTGAATG | CACAAATGAAGTGCCGCACA | Start: 1992 |
| Tm | 59.963 | 59.969 | End: 2206 |
| Size | 20 | 20 | 215 |
| Primer 3 details |
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| Sequence | TCTGGCCCCTCTAGAGTGTC | ACGCACCTTCACCATGCTAA | Start: 3338 |
| Tm | 60.033 | 59.964 | End: 3532 |
| Size | 20 | 20 | 195 |
| Primer 4 details |
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| Sequence | ATTGGGCTCTTTGGCGGTTA | ATCCCAAGTCTGCCCCATTG | Start: 1531 |
| Tm | 59.961 | 60.033 | End: 1718 |
| Size | 20 | 20 | 188 |
| Primer 5 details |
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| Sequence | CCACTCTTGCTGGGCAGTAA | ACGCACCTTCACCATGCTAA | Start: 3357 |
| Tm | 59.963 | 59.964 | End: 3532 |
| Size | 20 | 20 | 176 |
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