ssr_primer
Primer Details for TRINITY_DN70582_c2_g2_i3 |
|---|
| (T)16: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGGAGAATGTGAAGGCTGCT | AATCATCCAAGGGCGCAGAA | Start: 721 |
| Tm | 60.035 | 60.034 | End: 958 |
| Size | 20 | 20 | 238 |
| Primer 2 details |
|---|
| Sequence | GGGAGAATGTGAAGGCTGCT | AAATCATCCAAGGGCGCAGA | Start: 721 |
| Tm | 60.035 | 60.034 | End: 959 |
| Size | 20 | 20 | 239 |
| Primer 3 details |
|---|
| Sequence | TCTGCGCCCTTGGATGATTT | CGCCCTCTTCACCATCACTT | Start: 940 |
| Tm | 60.034 | 60.036 | End: 1073 |
| Size | 20 | 20 | 134 |
| Primer 4 details |
|---|
| Sequence | TTCTGCGCCCTTGGATGATT | CGCCCTCTTCACCATCACTT | Start: 939 |
| Tm | 60.034 | 60.036 | End: 1073 |
| Size | 20 | 20 | 135 |
| Primer 5 details |
|---|
| Sequence | CTCCAGTACTTTGCGGCTCA | TGGCCTGAAGCTCGAAATGT | Start: 66 |
| Tm | 60.037 | 59.963 | End: 295 |
| Size | 20 | 20 | 230 |
(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGGAGAATGTGAAGGCTGCT | AATCATCCAAGGGCGCAGAA | Start: 721 |
| Tm | 60.035 | 60.034 | End: 958 |
| Size | 20 | 20 | 238 |
| Primer 2 details |
|---|
| Sequence | GGGAGAATGTGAAGGCTGCT | AAATCATCCAAGGGCGCAGA | Start: 721 |
| Tm | 60.035 | 60.034 | End: 959 |
| Size | 20 | 20 | 239 |
| Primer 3 details |
|---|
| Sequence | TCTGCGCCCTTGGATGATTT | CGCCCTCTTCACCATCACTT | Start: 940 |
| Tm | 60.034 | 60.036 | End: 1073 |
| Size | 20 | 20 | 134 |
| Primer 4 details |
|---|
| Sequence | TTCTGCGCCCTTGGATGATT | CGCCCTCTTCACCATCACTT | Start: 939 |
| Tm | 60.034 | 60.036 | End: 1073 |
| Size | 20 | 20 | 135 |
| Primer 5 details |
|---|
| Sequence | CTCCAGTACTTTGCGGCTCA | TGGCCTGAAGCTCGAAATGT | Start: 66 |
| Tm | 60.037 | 59.963 | End: 295 |
| Size | 20 | 20 | 230 |
(T)28: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGGAGAATGTGAAGGCTGCT | AATCATCCAAGGGCGCAGAA | Start: 721 |
| Tm | 60.035 | 60.034 | End: 958 |
| Size | 20 | 20 | 238 |
| Primer 2 details |
|---|
| Sequence | GGGAGAATGTGAAGGCTGCT | AAATCATCCAAGGGCGCAGA | Start: 721 |
| Tm | 60.035 | 60.034 | End: 959 |
| Size | 20 | 20 | 239 |
| Primer 3 details |
|---|
| Sequence | TCTGCGCCCTTGGATGATTT | CGCCCTCTTCACCATCACTT | Start: 940 |
| Tm | 60.034 | 60.036 | End: 1073 |
| Size | 20 | 20 | 134 |
| Primer 4 details |
|---|
| Sequence | TTCTGCGCCCTTGGATGATT | CGCCCTCTTCACCATCACTT | Start: 939 |
| Tm | 60.034 | 60.036 | End: 1073 |
| Size | 20 | 20 | 135 |
| Primer 5 details |
|---|
| Sequence | CTCCAGTACTTTGCGGCTCA | TGGCCTGAAGCTCGAAATGT | Start: 66 |
| Tm | 60.037 | 59.963 | End: 295 |
| Size | 20 | 20 | 230 |
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