Primer Details for TRINITY_DN70542_c4_g1_i1 |
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| (A)13: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGTTCTGTTTGATAGAAATACGTTCA | GGCCTTATTCTCCCCGCAAA | Start: 1 |
| Tm | 57.173 | 60.395 | End: 155 |
| Size | 26 | 20 | 155 |
| Primer 2 details |
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| Sequence | AGTTCTGTTTGATAGAAATACGTTCA | TTTGGCCTTATTCTCCCCGC | Start: 1 |
| Tm | 57.173 | 60.395 | End: 158 |
| Size | 26 | 20 | 158 |
| Primer 3 details |
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| Sequence | AGTTCTGTTTGATAGAAATACGTTCAA | GGCCTTATTCTCCCCGCAAA | Start: 1 |
| Tm | 57.732 | 60.395 | End: 155 |
| Size | 27 | 20 | 155 |
| Primer 4 details |
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| Sequence | AGTTCTGTTTGATAGAAATACGTTCAA | TTTGGCCTTATTCTCCCCGC | Start: 1 |
| Tm | 57.732 | 60.395 | End: 158 |
| Size | 27 | 20 | 158 |
| Primer 5 details |
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| Sequence | AGTTCTGTTTGATAGAAATACGTTCA | GGCCTTATTCTCCCCGCAA | Start: 1 |
| Tm | 57.173 | 59.774 | End: 155 |
| Size | 26 | 19 | 155 |
