ssr_primer
Primer Details for TRINITY_DN70536_c5_g1_i7 |
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| (T)11aaattactgttctcattttgaagtatcagcgataaaaacggattaattgcgccaagaggcatatttaagccgattccttg(A)11: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGCTTGCATACAGGGTGGAG | ATGTCCATCGCTCGTCATCC | Start: 1083 |
| Tm | 60.035 | 59.967 | End: 1255 |
| Size | 20 | 20 | 173 |
| Primer 2 details |
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| Sequence | GGATGACGAGCGATGGACAT | TGTGCGATTCCAGCTTCAGT | Start: 1236 |
| Tm | 59.967 | 59.965 | End: 1423 |
| Size | 20 | 20 | 188 |
| Primer 3 details |
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| Sequence | GGATGACGAGCGATGGACAT | AACCACATGCAGAGTCTCCG | Start: 1236 |
| Tm | 59.967 | 60.037 | End: 1488 |
| Size | 20 | 20 | 253 |
| Primer 4 details |
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| Sequence | AAATGTTGTGTGCTTCCCGC | AAATCCTTTGGGCAGCTCGA | Start: 261 |
| Tm | 59.968 | 59.962 | End: 425 |
| Size | 20 | 20 | 165 |
| Primer 5 details |
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| Sequence | GGATGACGAGCGATGGACAT | ACTCTTGAGCGCAGGACTTC | Start: 1236 |
| Tm | 59.967 | 60.038 | End: 1396 |
| Size | 20 | 20 | 161 |
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