ssr_primer
Primer Details for TRINITY_DN70486_c5_g1_i10 |
|---|
| (A)11: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCTCCTGTCTTACGAGGGGA | CGGAATCTCGAACTCGGAGG | Start: 84 |
| Tm | 60.033 | 59.97 | End: 224 |
| Size | 20 | 20 | 141 |
| Primer 2 details |
|---|
| Sequence | CCTCCTGTCTTACGAGGGGA | CCGGAATCTCGAACTCGGAG | Start: 84 |
| Tm | 60.033 | 59.97 | End: 225 |
| Size | 20 | 20 | 142 |
| Primer 3 details |
|---|
| Sequence | TCGATTGGAAACCCCGTGAG | CGGAATCTCGAACTCGGAGG | Start: 25 |
| Tm | 60.037 | 59.97 | End: 224 |
| Size | 20 | 20 | 200 |
| Primer 4 details |
|---|
| Sequence | TCGATTGGAAACCCCGTGAG | CCGGAATCTCGAACTCGGAG | Start: 25 |
| Tm | 60.037 | 59.97 | End: 225 |
| Size | 20 | 20 | 201 |
| Primer 5 details |
|---|
| Sequence | TGGCGCAGAAATGGGAGATT | CAGCTTTCAAACCAGGTGGC | Start: 939 |
| Tm | 60.034 | 59.967 | End: 1146 |
| Size | 20 | 20 | 208 |
(A)20: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCTCCTGTCTTACGAGGGGA | CGGAATCTCGAACTCGGAGG | Start: 84 |
| Tm | 60.033 | 59.97 | End: 224 |
| Size | 20 | 20 | 141 |
| Primer 2 details |
|---|
| Sequence | CCTCCTGTCTTACGAGGGGA | CCGGAATCTCGAACTCGGAG | Start: 84 |
| Tm | 60.033 | 59.97 | End: 225 |
| Size | 20 | 20 | 142 |
| Primer 3 details |
|---|
| Sequence | TCGATTGGAAACCCCGTGAG | CGGAATCTCGAACTCGGAGG | Start: 25 |
| Tm | 60.037 | 59.97 | End: 224 |
| Size | 20 | 20 | 200 |
| Primer 4 details |
|---|
| Sequence | TCGATTGGAAACCCCGTGAG | CCGGAATCTCGAACTCGGAG | Start: 25 |
| Tm | 60.037 | 59.97 | End: 225 |
| Size | 20 | 20 | 201 |
| Primer 5 details |
|---|
| Sequence | TGGCGCAGAAATGGGAGATT | CAGCTTTCAAACCAGGTGGC | Start: 939 |
| Tm | 60.034 | 59.967 | End: 1146 |
| Size | 20 | 20 | 208 |
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