Primer Details for TRINITY_DN70476_c1_g1_i1 |
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| (T)11: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACGTTGATATTCTCATCATAGGGCA | TGGACAACCGTACATTGCGA | Start: 333 |
| Tm | 59.988 | 59.966 | End: 461 |
| Size | 25 | 20 | 129 |
| Primer 2 details |
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| Sequence | TTGTTACATTCGGGGCCCAA | TGCCCTATGATGAGAATATCAACGT | Start: 100 |
| Tm | 59.887 | 59.988 | End: 357 |
| Size | 20 | 25 | 258 |
| Primer 3 details |
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| Sequence | TTTGTTACATTCGGGGCCCA | TGCCCTATGATGAGAATATCAACGT | Start: 99 |
| Tm | 59.887 | 59.988 | End: 357 |
| Size | 20 | 25 | 259 |
| Primer 4 details |
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| Sequence | ACGTTGATATTCTCATCATAGGGCA | GTGGACAACCGTACATTGCG | Start: 333 |
| Tm | 59.988 | 59.835 | End: 462 |
| Size | 25 | 20 | 130 |
| Primer 5 details |
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| Sequence | TGTTACATTCGGGGCCCAAT | TGCCCTATGATGAGAATATCAACGT | Start: 101 |
| Tm | 59.667 | 59.988 | End: 357 |
| Size | 20 | 25 | 257 |
