ssr_primer
Primer Details for TRINITY_DN70365_c0_g1_i1 |
|---|
| (T)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CACGACGCTCTTCCGATCTT | ATCTGCCGCTCCAATTCGAA | Start: 38 |
| Tm | 60.179 | 60.108 | End: 182 |
| Size | 20 | 20 | 145 |
| Primer 2 details |
|---|
| Sequence | ACTCTTTCCCTACACGACGC | ATCTGCCGCTCCAATTCGAA | Start: 26 |
| Tm | 59.757 | 60.108 | End: 182 |
| Size | 20 | 20 | 157 |
| Primer 3 details |
|---|
| Sequence | GCGACCACCGAGATCTACAC | ATCTGCCGCTCCAATTCGAA | Start: 8 |
| Tm | 60.249 | 60.108 | End: 182 |
| Size | 20 | 20 | 175 |
| Primer 4 details |
|---|
| Sequence | CACGACGCTCTTCCGATCTT | CTGCCGCTCCAATTCGAAAC | Start: 38 |
| Tm | 60.179 | 60.179 | End: 180 |
| Size | 20 | 20 | 143 |
| Primer 5 details |
|---|
| Sequence | ACTCTTTCCCTACACGACGC | CTGCCGCTCCAATTCGAAAC | Start: 26 |
| Tm | 59.757 | 60.179 | End: 180 |
| Size | 20 | 20 | 155 |
(A)11t(A)11: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CACGACGCTCTTCCGATCTT | ATCTGCCGCTCCAATTCGAA | Start: 38 |
| Tm | 60.179 | 60.108 | End: 182 |
| Size | 20 | 20 | 145 |
| Primer 2 details |
|---|
| Sequence | ACTCTTTCCCTACACGACGC | ATCTGCCGCTCCAATTCGAA | Start: 26 |
| Tm | 59.757 | 60.108 | End: 182 |
| Size | 20 | 20 | 157 |
| Primer 3 details |
|---|
| Sequence | GCGACCACCGAGATCTACAC | ATCTGCCGCTCCAATTCGAA | Start: 8 |
| Tm | 60.249 | 60.108 | End: 182 |
| Size | 20 | 20 | 175 |
| Primer 4 details |
|---|
| Sequence | CACGACGCTCTTCCGATCTT | CTGCCGCTCCAATTCGAAAC | Start: 38 |
| Tm | 60.179 | 60.179 | End: 180 |
| Size | 20 | 20 | 143 |
| Primer 5 details |
|---|
| Sequence | ACTCTTTCCCTACACGACGC | CTGCCGCTCCAATTCGAAAC | Start: 26 |
| Tm | 59.757 | 60.179 | End: 180 |
| Size | 20 | 20 | 155 |
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