ssr_primer
Primer Details for TRINITY_DN70333_c11_g1_i1 |
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| (A)11cctggaaaaaagtcttgattatggtgtc(A)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGAGGGACCCTACGGTTAGT | TGGACGGCTGATTGTTGGAA | Start: 121 |
| Tm | 60.395 | 59.89 | End: 259 |
| Size | 20 | 20 | 139 |
| Primer 2 details |
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| Sequence | ACGAGGGACCCTACGGTTAG | TGGACGGCTGATTGTTGGAA | Start: 120 |
| Tm | 60.395 | 59.89 | End: 259 |
| Size | 20 | 20 | 140 |
| Primer 3 details |
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| Sequence | ATCCTATTGGTGGAAGCGGG | TGGACGGCTGATTGTTGGAA | Start: 50 |
| Tm | 59.526 | 59.89 | End: 259 |
| Size | 20 | 20 | 210 |
| Primer 4 details |
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| Sequence | CGGGTGGTTGGAATGGACAA | TGGACGGCTGATTGTTGGAA | Start: 66 |
| Tm | 60.539 | 59.89 | End: 259 |
| Size | 20 | 20 | 194 |
| Primer 5 details |
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| Sequence | AGACAAACTAGCGGCAACCC | TGGACGGCTGATTGTTGGAA | Start: 100 |
| Tm | 60.606 | 59.89 | End: 259 |
| Size | 20 | 20 | 160 |
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