ssr_primer
Primer Details for TRINITY_DN70322_c3_g3_i2 |
|---|
| (T)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ATGGGTGAAACGTGCAAACG | AGACTTCACTCCGGCAACAG | Start: 528 |
| Tm | 59.97 | 59.966 | End: 714 |
| Size | 20 | 20 | 187 |
| Primer 2 details |
|---|
| Sequence | GCCTCCCTCTTCTACATCGC | TGGCTGTGAGAGAGTGCTTG | Start: 1301 |
| Tm | 59.967 | 59.966 | End: 1578 |
| Size | 20 | 20 | 278 |
| Primer 3 details |
|---|
| Sequence | TGACGAATGCCAAGCTCTGT | GCGATGTAGAAGAGGGAGGC | Start: 1095 |
| Tm | 59.965 | 59.967 | End: 1320 |
| Size | 20 | 20 | 226 |
| Primer 4 details |
|---|
| Sequence | ATGGGTGAAACGTGCAAACG | TCACGCGACACAGATCGAAA | Start: 528 |
| Tm | 59.97 | 60.04 | End: 783 |
| Size | 20 | 20 | 256 |
| Primer 5 details |
|---|
| Sequence | ATGGGTGAAACGTGCAAACG | TTCACGCGACACAGATCGAA | Start: 528 |
| Tm | 59.97 | 60.04 | End: 784 |
| Size | 20 | 20 | 257 |
(T)25: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ATGGGTGAAACGTGCAAACG | AGACTTCACTCCGGCAACAG | Start: 528 |
| Tm | 59.97 | 59.966 | End: 714 |
| Size | 20 | 20 | 187 |
| Primer 2 details |
|---|
| Sequence | GCCTCCCTCTTCTACATCGC | TGGCTGTGAGAGAGTGCTTG | Start: 1301 |
| Tm | 59.967 | 59.966 | End: 1578 |
| Size | 20 | 20 | 278 |
| Primer 3 details |
|---|
| Sequence | TGACGAATGCCAAGCTCTGT | GCGATGTAGAAGAGGGAGGC | Start: 1095 |
| Tm | 59.965 | 59.967 | End: 1320 |
| Size | 20 | 20 | 226 |
| Primer 4 details |
|---|
| Sequence | ATGGGTGAAACGTGCAAACG | TCACGCGACACAGATCGAAA | Start: 528 |
| Tm | 59.97 | 60.04 | End: 783 |
| Size | 20 | 20 | 256 |
| Primer 5 details |
|---|
| Sequence | ATGGGTGAAACGTGCAAACG | TTCACGCGACACAGATCGAA | Start: 528 |
| Tm | 59.97 | 60.04 | End: 784 |
| Size | 20 | 20 | 257 |
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