ssr_primer
Primer Details for TRINITY_DN70322_c3_g1_i4 |
|---|
| (A)18: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCTGTGTCCATGAAGCAGCA | TGCTGCATGGGACTCATCAG | Start: 817 |
| Tm | 59.602 | 60.107 | End: 923 |
| Size | 20 | 20 | 107 |
| Primer 2 details |
|---|
| Sequence | CTGATGAGTCCCATGCAGCA | AGTGTTTGTGGGCTACGTGA | Start: 904 |
| Tm | 60.107 | 59.533 | End: 1026 |
| Size | 20 | 20 | 123 |
| Primer 3 details |
|---|
| Sequence | GTGTACCGCCTGATGAGTCC | AGTGTTTGTGGGCTACGTGA | Start: 895 |
| Tm | 60.179 | 59.533 | End: 1026 |
| Size | 20 | 20 | 132 |
| Primer 4 details |
|---|
| Sequence | GGTGTACCGCCTGATGAGTC | AGTGTTTGTGGGCTACGTGA | Start: 894 |
| Tm | 60.179 | 59.533 | End: 1026 |
| Size | 20 | 20 | 133 |
| Primer 5 details |
|---|
| Sequence | TCTGTGTCCATGAAGCAGCA | ACCGAGTCTTTGAGGGATGC | Start: 817 |
| Tm | 59.602 | 59.749 | End: 952 |
| Size | 20 | 20 | 136 |
(T)27: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCTGTGTCCATGAAGCAGCA | TGCTGCATGGGACTCATCAG | Start: 817 |
| Tm | 59.602 | 60.107 | End: 923 |
| Size | 20 | 20 | 107 |
| Primer 2 details |
|---|
| Sequence | CTGATGAGTCCCATGCAGCA | AGTGTTTGTGGGCTACGTGA | Start: 904 |
| Tm | 60.107 | 59.533 | End: 1026 |
| Size | 20 | 20 | 123 |
| Primer 3 details |
|---|
| Sequence | GTGTACCGCCTGATGAGTCC | AGTGTTTGTGGGCTACGTGA | Start: 895 |
| Tm | 60.179 | 59.533 | End: 1026 |
| Size | 20 | 20 | 132 |
| Primer 4 details |
|---|
| Sequence | GGTGTACCGCCTGATGAGTC | AGTGTTTGTGGGCTACGTGA | Start: 894 |
| Tm | 60.179 | 59.533 | End: 1026 |
| Size | 20 | 20 | 133 |
| Primer 5 details |
|---|
| Sequence | TCTGTGTCCATGAAGCAGCA | ACCGAGTCTTTGAGGGATGC | Start: 817 |
| Tm | 59.602 | 59.749 | End: 952 |
| Size | 20 | 20 | 136 |
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