ssr_primer
Primer Details for TRINITY_DN70303_c1_g1_i1 |
|---|
| (T)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGCTTTCGCCGAAACCACTA | GGTGCCTGGTTAGACCTTCC | Start: 713 |
| Tm | 59.966 | 60.035 | End: 843 |
| Size | 20 | 20 | 131 |
| Primer 2 details |
|---|
| Sequence | GCGAAAGAGAAACCTCCCCA | GGTGCCTGGTTAGACCTTCC | Start: 621 |
| Tm | 59.964 | 60.035 | End: 843 |
| Size | 20 | 20 | 223 |
| Primer 3 details |
|---|
| Sequence | AATGTAGCCTGTACTCGCGG | GGTGCCTGGTTAGACCTTCC | Start: 680 |
| Tm | 59.898 | 60.035 | End: 843 |
| Size | 20 | 20 | 164 |
| Primer 4 details |
|---|
| Sequence | AGCTTTCGCCGAAACCACTA | TCAGGTGCCTGGTTAGACCT | Start: 713 |
| Tm | 59.966 | 60.179 | End: 846 |
| Size | 20 | 20 | 134 |
| Primer 5 details |
|---|
| Sequence | GCGAAAGAGAAACCTCCCCA | TCAGGTGCCTGGTTAGACCT | Start: 621 |
| Tm | 59.964 | 60.179 | End: 846 |
| Size | 20 | 20 | 226 |
(T)15: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGCTTTCGCCGAAACCACTA | GGTGCCTGGTTAGACCTTCC | Start: 713 |
| Tm | 59.966 | 60.035 | End: 843 |
| Size | 20 | 20 | 131 |
| Primer 2 details |
|---|
| Sequence | GCGAAAGAGAAACCTCCCCA | GGTGCCTGGTTAGACCTTCC | Start: 621 |
| Tm | 59.964 | 60.035 | End: 843 |
| Size | 20 | 20 | 223 |
| Primer 3 details |
|---|
| Sequence | AATGTAGCCTGTACTCGCGG | GGTGCCTGGTTAGACCTTCC | Start: 680 |
| Tm | 59.898 | 60.035 | End: 843 |
| Size | 20 | 20 | 164 |
| Primer 4 details |
|---|
| Sequence | AGCTTTCGCCGAAACCACTA | TCAGGTGCCTGGTTAGACCT | Start: 713 |
| Tm | 59.966 | 60.179 | End: 846 |
| Size | 20 | 20 | 134 |
| Primer 5 details |
|---|
| Sequence | GCGAAAGAGAAACCTCCCCA | TCAGGTGCCTGGTTAGACCT | Start: 621 |
| Tm | 59.964 | 60.179 | End: 846 |
| Size | 20 | 20 | 226 |
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