ssr_primer
Primer Details for TRINITY_DN70298_c1_g1_i1 |
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| (T)23: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGAACGTTCTGTTACGCGAA | GTCCCTTTTCACGGATCCGA | Start: 77 |
| Tm | 59.228 | 59.752 | End: 350 |
| Size | 20 | 20 | 274 |
| Primer 2 details |
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| Sequence | ACGCGAACGGAAAAATGGAT | TCCGGAAGATGAGGTCCCTT | Start: 90 |
| Tm | 58.838 | 59.957 | End: 363 |
| Size | 20 | 20 | 274 |
| Primer 3 details |
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| Sequence | ACGCGAACGGAAAAATGGAT | TTCCGGAAGATGAGGTCCCT | Start: 90 |
| Tm | 58.838 | 59.957 | End: 364 |
| Size | 20 | 20 | 275 |
| Primer 4 details |
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| Sequence | CCGAACGTTCTGTTACGCGA | GTCCCTTTTCACGGATCCGA | Start: 76 |
| Tm | 60.998 | 59.752 | End: 350 |
| Size | 20 | 20 | 275 |
| Primer 5 details |
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| Sequence | CGTTCGCCCCCATCAAACTT | TCCGGAAGATGAGGTCCCTT | Start: 217 |
| Tm | 61.241 | 59.957 | End: 363 |
| Size | 20 | 20 | 147 |
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