Primer Details for TRINITY_DN70281_c4_g4_i1 | |||
|---|---|---|---|
| (T)15: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGGAGGAAACTTGGCAACGT | TTTCTACAAAATTCTCGCTTGCT | Start: 109 |
| Tm | 60.107 | 57.311 | End: 208 |
| Size | 20 | 23 | 100 |
| Primer 2 details | |||
| Sequence | TGGAGGAAACTTGGCAACGT | TTTCTACAAAATTCTCGCTTGCTT | Start: 109 |
| Tm | 60.107 | 57.928 | End: 208 |
| Size | 20 | 24 | 100 |
| Primer 3 details | |||
| Sequence | TGGAGGAAACTTGGCAACGT | TTTTCTACAAAATTCTCGCTTGCT | Start: 109 |
| Tm | 60.107 | 57.928 | End: 209 |
| Size | 20 | 24 | 101 |
| Primer 4 details | |||
| Sequence | TGGAGGAAACTTGGCAACGT | TTTTCTACAAAATTCTCGCTTGCTT | Start: 109 |
| Tm | 60.107 | 58.496 | End: 209 |
| Size | 20 | 25 | 101 |
| Primer 5 details | |||
| Sequence | GGAGGAAACTTGGCAACGTC | TTTTCTACAAAATTCTCGCTTGCT | Start: 110 |
| Tm | 59.408 | 57.928 | End: 209 |
| Size | 20 | 24 | 100 |
