ssr_primer
Primer Details for TRINITY_DN70242_c5_g2_i2 |
|---|
| (T)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGGGGAATGAGGGAGAGGTC | TGGTTGGCTGAAGGTGCTAG | Start: 77 |
| Tm | 60.031 | 59.963 | End: 196 |
| Size | 20 | 20 | 120 |
| Primer 2 details |
|---|
| Sequence | AGGGGAATGAGGGAGAGGTC | GGTTGGCTGAAGGTGCTAGT | Start: 77 |
| Tm | 60.031 | 59.963 | End: 195 |
| Size | 20 | 20 | 119 |
| Primer 3 details |
|---|
| Sequence | TTTCGACCGAAGTTAGGGCC | TGGTTGGCTGAAGGTGCTAG | Start: 27 |
| Tm | 60.037 | 59.963 | End: 196 |
| Size | 20 | 20 | 170 |
| Primer 4 details |
|---|
| Sequence | TTTCGACCGAAGTTAGGGCC | GGTTGGCTGAAGGTGCTAGT | Start: 27 |
| Tm | 60.037 | 59.963 | End: 195 |
| Size | 20 | 20 | 169 |
| Primer 5 details |
|---|
| Sequence | TACACGCGTCTGCAACTTCT | TGGTCAACCAATGGGCAAGT | Start: 1858 |
| Tm | 59.968 | 60.106 | End: 1976 |
| Size | 20 | 20 | 119 |
(A)10ggagaaagt(A)12: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGGGGAATGAGGGAGAGGTC | TGGTTGGCTGAAGGTGCTAG | Start: 77 |
| Tm | 60.031 | 59.963 | End: 196 |
| Size | 20 | 20 | 120 |
| Primer 2 details |
|---|
| Sequence | AGGGGAATGAGGGAGAGGTC | GGTTGGCTGAAGGTGCTAGT | Start: 77 |
| Tm | 60.031 | 59.963 | End: 195 |
| Size | 20 | 20 | 119 |
| Primer 3 details |
|---|
| Sequence | TTTCGACCGAAGTTAGGGCC | TGGTTGGCTGAAGGTGCTAG | Start: 27 |
| Tm | 60.037 | 59.963 | End: 196 |
| Size | 20 | 20 | 170 |
| Primer 4 details |
|---|
| Sequence | TTTCGACCGAAGTTAGGGCC | GGTTGGCTGAAGGTGCTAGT | Start: 27 |
| Tm | 60.037 | 59.963 | End: 195 |
| Size | 20 | 20 | 169 |
| Primer 5 details |
|---|
| Sequence | TACACGCGTCTGCAACTTCT | TGGTCAACCAATGGGCAAGT | Start: 1858 |
| Tm | 59.968 | 60.106 | End: 1976 |
| Size | 20 | 20 | 119 |
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