ssr_primer
Primer Details for TRINITY_DN70232_c5_g5_i1 |
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| (A)16: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGAAGGAAACGTCGGCCTAA | AAGACAACGTACGTGCCAGT | Start: 154 |
| Tm | 60.037 | 59.896 | End: 281 |
| Size | 20 | 20 | 128 |
| Primer 2 details |
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| Sequence | TGAGATCTTTCCACCGCGAG | AAGACAACGTACGTGCCAGT | Start: 107 |
| Tm | 59.827 | 59.896 | End: 281 |
| Size | 20 | 20 | 175 |
| Primer 3 details |
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| Sequence | GGAAGGAAACGTCGGCCTAA | AGACAACGTACGTGCCAGTC | Start: 154 |
| Tm | 60.037 | 60.318 | End: 280 |
| Size | 20 | 20 | 127 |
| Primer 4 details |
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| Sequence | CCAGATGGTCCCAGCTCAAA | AAGACAACGTACGTGCCAGT | Start: 80 |
| Tm | 59.671 | 59.896 | End: 281 |
| Size | 20 | 20 | 202 |
| Primer 5 details |
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| Sequence | GGAAGGAAACGTCGGCCTAA | CCCTGCAGGCTATAGGCATT | Start: 154 |
| Tm | 60.037 | 59.597 | End: 408 |
| Size | 20 | 20 | 255 |
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