ssr_primer
Primer Details for TRINITY_DN70201_c9_g1_i1 |
|---|
| (T)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CAGACGTTGCCAGATTCCCT | GAAGGAAGAATCGAGCGCCT | Start: 310 |
| Tm | 60.036 | 60.179 | End: 515 |
| Size | 20 | 20 | 206 |
| Primer 2 details |
|---|
| Sequence | AGGCGCTCGATTCTTCCTTC | ACTTTCACTCCTGGCAGCTC | Start: 496 |
| Tm | 60.179 | 59.964 | End: 663 |
| Size | 20 | 20 | 168 |
| Primer 3 details |
|---|
| Sequence | TCAGACGTTGCCAGATTCCC | GAAGGAAGAATCGAGCGCCT | Start: 309 |
| Tm | 60.036 | 60.179 | End: 515 |
| Size | 20 | 20 | 207 |
| Primer 4 details |
|---|
| Sequence | CAGACGTTGCCAGATTCCCT | GAATCGAGCGCCTTTTGAGC | Start: 310 |
| Tm | 60.036 | 60.248 | End: 508 |
| Size | 20 | 20 | 199 |
| Primer 5 details |
|---|
| Sequence | GCTCAAAAGGCGCTCGATTC | ACTTTCACTCCTGGCAGCTC | Start: 489 |
| Tm | 60.248 | 59.964 | End: 663 |
| Size | 20 | 20 | 175 |
(T)16: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CAGACGTTGCCAGATTCCCT | GAAGGAAGAATCGAGCGCCT | Start: 310 |
| Tm | 60.036 | 60.179 | End: 515 |
| Size | 20 | 20 | 206 |
| Primer 2 details |
|---|
| Sequence | AGGCGCTCGATTCTTCCTTC | ACTTTCACTCCTGGCAGCTC | Start: 496 |
| Tm | 60.179 | 59.964 | End: 663 |
| Size | 20 | 20 | 168 |
| Primer 3 details |
|---|
| Sequence | TCAGACGTTGCCAGATTCCC | GAAGGAAGAATCGAGCGCCT | Start: 309 |
| Tm | 60.036 | 60.179 | End: 515 |
| Size | 20 | 20 | 207 |
| Primer 4 details |
|---|
| Sequence | CAGACGTTGCCAGATTCCCT | GAATCGAGCGCCTTTTGAGC | Start: 310 |
| Tm | 60.036 | 60.248 | End: 508 |
| Size | 20 | 20 | 199 |
| Primer 5 details |
|---|
| Sequence | GCTCAAAAGGCGCTCGATTC | ACTTTCACTCCTGGCAGCTC | Start: 489 |
| Tm | 60.248 | 59.964 | End: 663 |
| Size | 20 | 20 | 175 |
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