ssr_primer
Primer Details for TRINITY_DN70158_c3_g1_i3 |
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| (A)17: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GTCAGAGTGGCGGCTAGTTT | AAGCAGATTGCCTCGATGGA | Start: 534 |
| Tm | 60.037 | 59.457 | End: 665 |
| Size | 20 | 20 | 132 |
| Primer 2 details |
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| Sequence | GTCAGAGTGGCGGCTAGTTT | AGCAGATTGCCTCGATGGAT | Start: 534 |
| Tm | 60.037 | 59.236 | End: 664 |
| Size | 20 | 20 | 131 |
| Primer 3 details |
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| Sequence | CAAGTCAGAGTGGCGGCTAG | AAGCAGATTGCCTCGATGGA | Start: 531 |
| Tm | 60.459 | 59.457 | End: 665 |
| Size | 20 | 20 | 135 |
| Primer 4 details |
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| Sequence | GTCAGAGTGGCGGCTAGTTT | AAAGCAGATTGCCTCGATGGA | Start: 534 |
| Tm | 60.037 | 60.065 | End: 666 |
| Size | 20 | 21 | 133 |
| Primer 5 details |
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| Sequence | GTCAGAGTGGCGGCTAGTTT | CCCAGGTAAAGCAGATTGCC | Start: 534 |
| Tm | 60.037 | 58.894 | End: 673 |
| Size | 20 | 20 | 140 |
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