ssr_primer
Primer Details for TRINITY_DN70154_c3_g1_i5 |
|---|
| (C)24: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TATCGGCACTTGTGCAACCT | TTGGGCAGGAGAAGCAAGAG | Start: 1497 |
| Tm | 59.964 | 59.963 | End: 1662 |
| Size | 20 | 20 | 166 |
| Primer 2 details |
|---|
| Sequence | TGAAAATCTGTGGCTGCCCA | ACCCTAAAAAGATGGGCGGG | Start: 3208 |
| Tm | 60.179 | 60.034 | End: 3405 |
| Size | 20 | 20 | 198 |
| Primer 3 details |
|---|
| Sequence | CTTGGCCACCCCACAATTTG | TGGGCAGCCACAGATTTTCA | Start: 3084 |
| Tm | 59.965 | 60.179 | End: 3227 |
| Size | 20 | 20 | 144 |
| Primer 4 details |
|---|
| Sequence | ACTTTCTCACCGCCTCCTTG | TGGGCAGCCACAGATTTTCA | Start: 3068 |
| Tm | 59.965 | 60.179 | End: 3227 |
| Size | 20 | 20 | 160 |
| Primer 5 details |
|---|
| Sequence | CTTGAGGCCGGGTGTAATGT | CACAGCACACAGAGGACCAA | Start: 231 |
| Tm | 60.035 | 60.179 | End: 410 |
| Size | 20 | 20 | 180 |
(A)23: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TATCGGCACTTGTGCAACCT | TTGGGCAGGAGAAGCAAGAG | Start: 1497 |
| Tm | 59.964 | 59.963 | End: 1662 |
| Size | 20 | 20 | 166 |
| Primer 2 details |
|---|
| Sequence | TGAAAATCTGTGGCTGCCCA | ACCCTAAAAAGATGGGCGGG | Start: 3208 |
| Tm | 60.179 | 60.034 | End: 3405 |
| Size | 20 | 20 | 198 |
| Primer 3 details |
|---|
| Sequence | CTTGGCCACCCCACAATTTG | TGGGCAGCCACAGATTTTCA | Start: 3084 |
| Tm | 59.965 | 60.179 | End: 3227 |
| Size | 20 | 20 | 144 |
| Primer 4 details |
|---|
| Sequence | ACTTTCTCACCGCCTCCTTG | TGGGCAGCCACAGATTTTCA | Start: 3068 |
| Tm | 59.965 | 60.179 | End: 3227 |
| Size | 20 | 20 | 160 |
| Primer 5 details |
|---|
| Sequence | CTTGAGGCCGGGTGTAATGT | CACAGCACACAGAGGACCAA | Start: 231 |
| Tm | 60.035 | 60.179 | End: 410 |
| Size | 20 | 20 | 180 |
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