ssr_primer
Primer Details for TRINITY_DN70150_c4_g1_i1 |
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| (C)11tgcacggccaaaaaa(C)14(A)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GTTGACACCTTTTTCGGCCC | TCTCCGCTGAGAAGATGACA | Start: 27 |
| Tm | 59.968 | 58.161 | End: 171 |
| Size | 20 | 20 | 145 |
| Primer 2 details |
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| Sequence | GCGCCAAGTTGACACCTTTT | TCTCCGCTGAGAAGATGACA | Start: 20 |
| Tm | 59.898 | 58.161 | End: 171 |
| Size | 20 | 20 | 152 |
| Primer 3 details |
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| Sequence | TTTTGCGCCAAGTTGACACC | TCTCCGCTGAGAAGATGACA | Start: 16 |
| Tm | 60.179 | 58.161 | End: 171 |
| Size | 20 | 20 | 156 |
| Primer 4 details |
|---|
| Sequence | GTTGACACCTTTTTCGGCCC | TCTCCGCTGAGAAGATGACAA | Start: 27 |
| Tm | 59.968 | 58.829 | End: 171 |
| Size | 20 | 21 | 145 |
| Primer 5 details |
|---|
| Sequence | GTTGACACCTTTTTCGGCCC | TCCGCTGAGAAGATGACAATGT | Start: 27 |
| Tm | 59.968 | 59.765 | End: 169 |
| Size | 20 | 22 | 143 |
(A)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GTTGACACCTTTTTCGGCCC | TCTCCGCTGAGAAGATGACA | Start: 27 |
| Tm | 59.968 | 58.161 | End: 171 |
| Size | 20 | 20 | 145 |
| Primer 2 details |
|---|
| Sequence | GCGCCAAGTTGACACCTTTT | TCTCCGCTGAGAAGATGACA | Start: 20 |
| Tm | 59.898 | 58.161 | End: 171 |
| Size | 20 | 20 | 152 |
| Primer 3 details |
|---|
| Sequence | TTTTGCGCCAAGTTGACACC | TCTCCGCTGAGAAGATGACA | Start: 16 |
| Tm | 60.179 | 58.161 | End: 171 |
| Size | 20 | 20 | 156 |
| Primer 4 details |
|---|
| Sequence | GTTGACACCTTTTTCGGCCC | TCTCCGCTGAGAAGATGACAA | Start: 27 |
| Tm | 59.968 | 58.829 | End: 171 |
| Size | 20 | 21 | 145 |
| Primer 5 details |
|---|
| Sequence | GTTGACACCTTTTTCGGCCC | TCCGCTGAGAAGATGACAATGT | Start: 27 |
| Tm | 59.968 | 59.765 | End: 169 |
| Size | 20 | 22 | 143 |
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