ssr_primer
Primer Details for TRINITY_DN70133_c0_g1_i1 |
|---|
| (T)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACGCTCGTGCAAGTTAGGTT | CTCTGGTGGGGAATATCGGC | Start: 110 |
| Tm | 60.25 | 59.965 | End: 388 |
| Size | 20 | 20 | 279 |
| Primer 2 details |
|---|
| Sequence | GCCGATATTCCCCACCAGAG | GCTTGCCATCTGGAGAAAGC | Start: 369 |
| Tm | 59.965 | 59.545 | End: 615 |
| Size | 20 | 20 | 247 |
| Primer 3 details |
|---|
| Sequence | TTGAAATTCTCCGTCGCCGA | GCTTGCCATCTGGAGAAAGC | Start: 354 |
| Tm | 60.038 | 59.545 | End: 615 |
| Size | 20 | 20 | 262 |
| Primer 4 details |
|---|
| Sequence | TGAAATTCTCCGTCGCCGAT | GCTTGCCATCTGGAGAAAGC | Start: 355 |
| Tm | 59.825 | 59.545 | End: 615 |
| Size | 20 | 20 | 261 |
| Primer 5 details |
|---|
| Sequence | TACGCTCGTGCAAGTTAGGT | CTCTGGTGGGGAATATCGGC | Start: 109 |
| Tm | 59.4 | 59.965 | End: 388 |
| Size | 20 | 20 | 280 |
(T)11: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACGCTCGTGCAAGTTAGGTT | CTCTGGTGGGGAATATCGGC | Start: 110 |
| Tm | 60.25 | 59.965 | End: 388 |
| Size | 20 | 20 | 279 |
| Primer 2 details |
|---|
| Sequence | GCCGATATTCCCCACCAGAG | GCTTGCCATCTGGAGAAAGC | Start: 369 |
| Tm | 59.965 | 59.545 | End: 615 |
| Size | 20 | 20 | 247 |
| Primer 3 details |
|---|
| Sequence | TTGAAATTCTCCGTCGCCGA | GCTTGCCATCTGGAGAAAGC | Start: 354 |
| Tm | 60.038 | 59.545 | End: 615 |
| Size | 20 | 20 | 262 |
| Primer 4 details |
|---|
| Sequence | TGAAATTCTCCGTCGCCGAT | GCTTGCCATCTGGAGAAAGC | Start: 355 |
| Tm | 59.825 | 59.545 | End: 615 |
| Size | 20 | 20 | 261 |
| Primer 5 details |
|---|
| Sequence | TACGCTCGTGCAAGTTAGGT | CTCTGGTGGGGAATATCGGC | Start: 109 |
| Tm | 59.4 | 59.965 | End: 388 |
| Size | 20 | 20 | 280 |
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