ssr_primer
Primer Details for TRINITY_DN70118_c5_g4_i1 |
|---|
| (T)12(A)12: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGACGCGACTAGACGATTCC | CAGAGGCGGCTCATTCTACC | Start: 411 |
| Tm | 60.317 | 60.25 | End: 563 |
| Size | 20 | 20 | 153 |
| Primer 2 details |
|---|
| Sequence | GGACGCGACTAGACGATTCC | CCAGAGGCGGCTCATTCTAC | Start: 411 |
| Tm | 60.317 | 60.25 | End: 564 |
| Size | 20 | 20 | 154 |
| Primer 3 details |
|---|
| Sequence | TTTTCGGACGCGACTAGACG | CAGAGGCGGCTCATTCTACC | Start: 406 |
| Tm | 60.453 | 60.25 | End: 563 |
| Size | 20 | 20 | 158 |
| Primer 4 details |
|---|
| Sequence | TTTTCGGACGCGACTAGACG | CCAGAGGCGGCTCATTCTAC | Start: 406 |
| Tm | 60.453 | 60.25 | End: 564 |
| Size | 20 | 20 | 159 |
| Primer 5 details |
|---|
| Sequence | GGACGCGACTAGACGATTCC | GGCGGCTCATTCTACCACAA | Start: 411 |
| Tm | 60.317 | 60.392 | End: 559 |
| Size | 20 | 20 | 149 |
(A)10taaaaataaaaaat(A)11: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGACGCGACTAGACGATTCC | CAGAGGCGGCTCATTCTACC | Start: 411 |
| Tm | 60.317 | 60.25 | End: 563 |
| Size | 20 | 20 | 153 |
| Primer 2 details |
|---|
| Sequence | GGACGCGACTAGACGATTCC | CCAGAGGCGGCTCATTCTAC | Start: 411 |
| Tm | 60.317 | 60.25 | End: 564 |
| Size | 20 | 20 | 154 |
| Primer 3 details |
|---|
| Sequence | TTTTCGGACGCGACTAGACG | CAGAGGCGGCTCATTCTACC | Start: 406 |
| Tm | 60.453 | 60.25 | End: 563 |
| Size | 20 | 20 | 158 |
| Primer 4 details |
|---|
| Sequence | TTTTCGGACGCGACTAGACG | CCAGAGGCGGCTCATTCTAC | Start: 406 |
| Tm | 60.453 | 60.25 | End: 564 |
| Size | 20 | 20 | 159 |
| Primer 5 details |
|---|
| Sequence | GGACGCGACTAGACGATTCC | GGCGGCTCATTCTACCACAA | Start: 411 |
| Tm | 60.317 | 60.392 | End: 559 |
| Size | 20 | 20 | 149 |
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