ssr_primer
Primer Details for TRINITY_DN70100_c5_g1_i1 |
|---|
| (A)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGCCGGTGCATAGGAGTTTT | TGATGGATGTCCGTGTTGCA | Start: 264 |
| Tm | 59.962 | 59.964 | End: 523 |
| Size | 20 | 20 | 260 |
| Primer 2 details |
|---|
| Sequence | TGCCGGTGCATAGGAGTTTT | ATTGAAGGGGCTATGAGGCG | Start: 264 |
| Tm | 59.962 | 59.891 | End: 495 |
| Size | 20 | 20 | 232 |
| Primer 3 details |
|---|
| Sequence | TCTCTCCCGCATCCTAGCAT | TGATGGATGTCCGTGTTGCA | Start: 412 |
| Tm | 60.179 | 59.964 | End: 523 |
| Size | 20 | 20 | 112 |
| Primer 4 details |
|---|
| Sequence | TGCCGGTGCATAGGAGTTTT | ATGCTAGGATGCGGGAGAGA | Start: 264 |
| Tm | 59.962 | 60.179 | End: 431 |
| Size | 20 | 20 | 168 |
| Primer 5 details |
|---|
| Sequence | GAGGCTTGCCAAAAACACGT | CGGCTTGGGATTTCTAGGGG | Start: 42 |
| Tm | 59.898 | 60.179 | End: 223 |
| Size | 20 | 20 | 182 |
(A)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGCCGGTGCATAGGAGTTTT | TGATGGATGTCCGTGTTGCA | Start: 264 |
| Tm | 59.962 | 59.964 | End: 523 |
| Size | 20 | 20 | 260 |
| Primer 2 details |
|---|
| Sequence | TGCCGGTGCATAGGAGTTTT | ATTGAAGGGGCTATGAGGCG | Start: 264 |
| Tm | 59.962 | 59.891 | End: 495 |
| Size | 20 | 20 | 232 |
| Primer 3 details |
|---|
| Sequence | TCTCTCCCGCATCCTAGCAT | TGATGGATGTCCGTGTTGCA | Start: 412 |
| Tm | 60.179 | 59.964 | End: 523 |
| Size | 20 | 20 | 112 |
| Primer 4 details |
|---|
| Sequence | TGCCGGTGCATAGGAGTTTT | ATGCTAGGATGCGGGAGAGA | Start: 264 |
| Tm | 59.962 | 60.179 | End: 431 |
| Size | 20 | 20 | 168 |
| Primer 5 details |
|---|
| Sequence | GAGGCTTGCCAAAAACACGT | CGGCTTGGGATTTCTAGGGG | Start: 42 |
| Tm | 59.898 | 60.179 | End: 223 |
| Size | 20 | 20 | 182 |
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