ssr_primer
Primer Details for TRINITY_DN70053_c2_g1_i1 |
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| (G)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGGTGATATTCTCCTCCGCC | GGAGCCTTCCCCGGAAAATT | Start: 48 |
| Tm | 60.038 | 60.324 | End: 287 |
| Size | 20 | 20 | 240 |
| Primer 2 details |
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| Sequence | TGATATTCTCCTCCGCCCCT | GGAGCCTTCCCCGGAAAATT | Start: 51 |
| Tm | 59.809 | 60.324 | End: 287 |
| Size | 20 | 20 | 237 |
| Primer 3 details |
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| Sequence | AATTTTCCGGGGAAGGCTCC | GTGCGACTCCGTAAACCTCT | Start: 268 |
| Tm | 60.324 | 59.757 | End: 488 |
| Size | 20 | 20 | 221 |
| Primer 4 details |
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| Sequence | AATTTTCCGGGGAAGGCTCC | AGTGCGACTCCGTAAACCTC | Start: 268 |
| Tm | 60.324 | 59.757 | End: 489 |
| Size | 20 | 20 | 222 |
| Primer 5 details |
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| Sequence | GCCCCTTTTCGCCTTTTCTC | GGAGCCTTCCCCGGAAAATT | Start: 65 |
| Tm | 59.756 | 60.324 | End: 287 |
| Size | 20 | 20 | 223 |
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