ssr_primer
Primer Details for TRINITY_DN69918_c4_g6_i2 |
|---|
| (A)24: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCGTTGGTATCGAAGTGAGCA | TGCACAAGAGGCTAATAGTCAGT | Start: 153 |
| Tm | 59.458 | 59.484 | End: 252 |
| Size | 21 | 23 | 100 |
| Primer 2 details |
|---|
| Sequence | TCGTTGGTATCGAAGTGAGCA | TCTGCACAAGAGGCTAATAGTCA | Start: 153 |
| Tm | 59.458 | 59.231 | End: 254 |
| Size | 21 | 23 | 102 |
| Primer 3 details |
|---|
| Sequence | TCGTTGGTATCGAAGTGAGCAA | TGCACAAGAGGCTAATAGTCAGT | Start: 153 |
| Tm | 60.03 | 59.484 | End: 252 |
| Size | 22 | 23 | 100 |
| Primer 4 details |
|---|
| Sequence | TTCGTTGGTATCGAAGTGAGCA | TGCACAAGAGGCTAATAGTCAGT | Start: 152 |
| Tm | 60.03 | 59.484 | End: 252 |
| Size | 22 | 23 | 101 |
| Primer 5 details |
|---|
| Sequence | TCGTTGGTATCGAAGTGAGCA | CTGCACAAGAGGCTAATAGTCAG | Start: 153 |
| Tm | 59.458 | 58.882 | End: 253 |
| Size | 21 | 23 | 101 |
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