ssr_primer
Primer Details for TRINITY_DN69918_c4_g4_i1 |
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| (A)20: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCCGTACCGACTTACGTCAT | AAGGAGAGAGATTGCGGTGC | Start: 142 |
| Tm | 59.901 | 60.108 | End: 284 |
| Size | 20 | 20 | 143 |
| Primer 2 details |
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| Sequence | TGTAAAAACCCCTGTCGCCA | AGAGCAACGGAGGAGGATCT | Start: 292 |
| Tm | 59.817 | 60.033 | End: 555 |
| Size | 20 | 20 | 264 |
| Primer 3 details |
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| Sequence | GCCGTACCGACTTACGTCAT | ATGGTAGCTAAGTGCCGCAG | Start: 142 |
| Tm | 59.901 | 60.179 | End: 263 |
| Size | 20 | 20 | 122 |
| Primer 4 details |
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| Sequence | GCCGTACCGACTTACGTCAT | TGGCGACAGGGGTTTTTACA | Start: 142 |
| Tm | 59.901 | 59.817 | End: 311 |
| Size | 20 | 20 | 170 |
| Primer 5 details |
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| Sequence | TGTAAAAACCCCTGTCGCCA | GCTAAGAGCAACGGAGGAGG | Start: 292 |
| Tm | 59.817 | 60.179 | End: 559 |
| Size | 20 | 20 | 268 |
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