ssr_primer
Primer Details for TRINITY_DN69918_c4_g1_i1 |
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| (A)27: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGGGGGTTGTCTTGACTCTC | AGGTGTTTGCATTTTCGGGC | Start: 165 |
| Tm | 60.037 | 59.967 | End: 444 |
| Size | 20 | 20 | 280 |
| Primer 2 details |
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| Sequence | CGGGGGTTGTCTTGACTCTC | ACGTAGGTCGGTACAGACGA | Start: 165 |
| Tm | 60.037 | 60.037 | End: 385 |
| Size | 20 | 20 | 221 |
| Primer 3 details |
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| Sequence | TTTGTGACTCCATGAGGGGC | ACGTAGGTCGGTACAGACGA | Start: 143 |
| Tm | 59.962 | 60.037 | End: 385 |
| Size | 20 | 20 | 243 |
| Primer 4 details |
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| Sequence | ATGCCCGTTTGTGACTCCAT | ACGTAGGTCGGTACAGACGA | Start: 136 |
| Tm | 59.962 | 60.037 | End: 385 |
| Size | 20 | 20 | 250 |
| Primer 5 details |
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| Sequence | TGGACTGGAGGATTACGGGT | GAGAGTCAAGACAACCCCCG | Start: 18 |
| Tm | 59.958 | 60.037 | End: 184 |
| Size | 20 | 20 | 167 |
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