ssr_primer
Primer Details for TRINITY_DN69871_c5_g4_i1 |
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| (T)18: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGCCGCAATGAACATGGAAA | GACAGCCTTCCATCGTGACA | Start: 1127 |
| Tm | 60.109 | 60.037 | End: 1239 |
| Size | 20 | 20 | 113 |
| Primer 2 details |
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| Sequence | CACTCGCTCTCTCCCTCTCT | TCTCACCTCGCATCCAATCG | Start: 269 |
| Tm | 60.107 | 59.897 | End: 455 |
| Size | 20 | 20 | 187 |
| Primer 3 details |
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| Sequence | CTGCCCTTGTGCATCCTACA | ATGCTGCGAAGACGTACCTC | Start: 1995 |
| Tm | 60.035 | 60.179 | End: 2158 |
| Size | 20 | 20 | 164 |
| Primer 4 details |
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| Sequence | GAGGTACGTCTTCGCAGCAT | TTCTTCCCCGTCCTTTTCCG | Start: 2139 |
| Tm | 60.179 | 59.964 | End: 2379 |
| Size | 20 | 20 | 241 |
| Primer 5 details |
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| Sequence | TACAGTAGCTGCCCTTGTGC | ATGCTGCGAAGACGTACCTC | Start: 1987 |
| Tm | 60.036 | 60.179 | End: 2158 |
| Size | 20 | 20 | 172 |
(G)14: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGCCGCAATGAACATGGAAA | GACAGCCTTCCATCGTGACA | Start: 1127 |
| Tm | 60.109 | 60.037 | End: 1239 |
| Size | 20 | 20 | 113 |
| Primer 2 details |
|---|
| Sequence | CACTCGCTCTCTCCCTCTCT | TCTCACCTCGCATCCAATCG | Start: 269 |
| Tm | 60.107 | 59.897 | End: 455 |
| Size | 20 | 20 | 187 |
| Primer 3 details |
|---|
| Sequence | CTGCCCTTGTGCATCCTACA | ATGCTGCGAAGACGTACCTC | Start: 1995 |
| Tm | 60.035 | 60.179 | End: 2158 |
| Size | 20 | 20 | 164 |
| Primer 4 details |
|---|
| Sequence | GAGGTACGTCTTCGCAGCAT | TTCTTCCCCGTCCTTTTCCG | Start: 2139 |
| Tm | 60.179 | 59.964 | End: 2379 |
| Size | 20 | 20 | 241 |
| Primer 5 details |
|---|
| Sequence | TACAGTAGCTGCCCTTGTGC | ATGCTGCGAAGACGTACCTC | Start: 1987 |
| Tm | 60.036 | 60.179 | End: 2158 |
| Size | 20 | 20 | 172 |
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