ssr_primer
Primer Details for TRINITY_DN69861_c2_g2_i2 |
|---|
| (A)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGGGCCTCTGATGTGTGAAA | GGAGGTCCACTTCAAAGCGA | Start: 506 |
| Tm | 59.962 | 59.965 | End: 618 |
| Size | 20 | 20 | 113 |
| Primer 2 details |
|---|
| Sequence | ATATTTGCAGGGGCTGGGAC | TTTCACACATCAGAGGCCCC | Start: 266 |
| Tm | 60.106 | 59.962 | End: 525 |
| Size | 20 | 20 | 260 |
| Primer 3 details |
|---|
| Sequence | AACAAGAGAGGGGACGGAGA | GGAGGTCCACTTCAAAGCGA | Start: 376 |
| Tm | 59.885 | 59.965 | End: 618 |
| Size | 20 | 20 | 243 |
| Primer 4 details |
|---|
| Sequence | AACAAGAGAGGGGACGGAGA | TTTCACACATCAGAGGCCCC | Start: 376 |
| Tm | 59.885 | 59.962 | End: 525 |
| Size | 20 | 20 | 150 |
| Primer 5 details |
|---|
| Sequence | GATGTGTGAAATTGCCCCCG | GGAGGTCCACTTCAAAGCGA | Start: 515 |
| Tm | 59.827 | 59.965 | End: 618 |
| Size | 20 | 20 | 104 |
(C)12: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGGGCCTCTGATGTGTGAAA | GGAGGTCCACTTCAAAGCGA | Start: 506 |
| Tm | 59.962 | 59.965 | End: 618 |
| Size | 20 | 20 | 113 |
| Primer 2 details |
|---|
| Sequence | ATATTTGCAGGGGCTGGGAC | TTTCACACATCAGAGGCCCC | Start: 266 |
| Tm | 60.106 | 59.962 | End: 525 |
| Size | 20 | 20 | 260 |
| Primer 3 details |
|---|
| Sequence | AACAAGAGAGGGGACGGAGA | GGAGGTCCACTTCAAAGCGA | Start: 376 |
| Tm | 59.885 | 59.965 | End: 618 |
| Size | 20 | 20 | 243 |
| Primer 4 details |
|---|
| Sequence | AACAAGAGAGGGGACGGAGA | TTTCACACATCAGAGGCCCC | Start: 376 |
| Tm | 59.885 | 59.962 | End: 525 |
| Size | 20 | 20 | 150 |
| Primer 5 details |
|---|
| Sequence | GATGTGTGAAATTGCCCCCG | GGAGGTCCACTTCAAAGCGA | Start: 515 |
| Tm | 59.827 | 59.965 | End: 618 |
| Size | 20 | 20 | 104 |
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