Primer Details for TRINITY_DN69853_c7_g1_i1 | |||
|---|---|---|---|
| (A)17: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGCTGGTACCATAGAGTGCA | TTTTTAGCCATGTTTTGCTACCT | Start: 116 |
| Tm | 59.169 | 57.2 | End: 226 |
| Size | 20 | 23 | 111 |
| Primer 2 details | |||
| Sequence | TGGCTGGTACCATAGAGTGC | TTTTTAGCCATGTTTTGCTACCT | Start: 115 |
| Tm | 59.169 | 57.2 | End: 226 |
| Size | 20 | 23 | 112 |
| Primer 3 details | |||
| Sequence | TTGGCTGGTACCATAGAGTGC | TTTGCTACCTATTTAATGTGGCCT | Start: 114 |
| Tm | 59.79 | 58.485 | End: 213 |
| Size | 21 | 24 | 100 |
| Primer 4 details | |||
| Sequence | TGGCTGGTACCATAGAGTGC | TTTTGCTACCTATTTAATGTGGCCT | Start: 115 |
| Tm | 59.169 | 59.045 | End: 214 |
| Size | 20 | 25 | 100 |
| Primer 5 details | |||
| Sequence | GGCTGGTACCATAGAGTGCA | GTTTTGCTACCTATTTAATGTGGCC | Start: 116 |
| Tm | 59.169 | 58.96 | End: 215 |
| Size | 20 | 25 | 100 |
