Primer Details for TRINITY_DN69853_c2_g1_i1 |
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| (T)11: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGGTCTGGGAAAGTCCACAC | TGGAGGCTGTAATTATCATTTGTGTG | Start: 5 |
| Tm | 59.528 | 59.905 | End: 238 |
| Size | 20 | 26 | 234 |
| Primer 2 details |
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| Sequence | TGGTCTGGGAAAGTCCACAC | AGGCTGTAATTATCATTTGTGTGGA | Start: 5 |
| Tm | 59.528 | 58.813 | End: 235 |
| Size | 20 | 25 | 231 |
| Primer 3 details |
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| Sequence | TGGTCTGGGAAAGTCCACAC | TGGAGGCTGTAATTATCATTTGTGT | Start: 5 |
| Tm | 59.528 | 58.813 | End: 238 |
| Size | 20 | 25 | 234 |
| Primer 4 details |
|---|
| Sequence | TGGTCTGGGAAAGTCCACAC | GGCTGTAATTATCATTTGTGTGGAGA | Start: 5 |
| Tm | 59.528 | 59.681 | End: 234 |
| Size | 20 | 26 | 230 |
| Primer 5 details |
|---|
| Sequence | TGGTCTGGGAAAGTCCACAC | GAGGCTGTAATTATCATTTGTGTGGA | Start: 5 |
| Tm | 59.528 | 59.681 | End: 236 |
| Size | 20 | 26 | 232 |
