ssr_primer
Primer Details for TRINITY_DN69842_c4_g4_i3 |
|---|
| (T)15: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GAAAACGTGCACCATGGCAT | ACCGACGTCTTCTGGAGAGA | Start: 299 |
| Tm | 60.038 | 59.964 | End: 402 |
| Size | 20 | 20 | 104 |
| Primer 2 details |
|---|
| Sequence | GCCGTTTGCCCTCTTGTTTT | TACAGCACTTGCCCTGCTAC | Start: 1163 |
| Tm | 59.896 | 60.036 | End: 1335 |
| Size | 20 | 20 | 173 |
| Primer 3 details |
|---|
| Sequence | TACCGCGAGTTTGGCATCAT | ATGCCATGGTGCACGTTTTC | Start: 107 |
| Tm | 60.108 | 60.038 | End: 318 |
| Size | 20 | 20 | 212 |
| Primer 4 details |
|---|
| Sequence | CCGCGAGTTTGGCATCATTT | ATGCCATGGTGCACGTTTTC | Start: 109 |
| Tm | 59.829 | 60.038 | End: 318 |
| Size | 20 | 20 | 210 |
| Primer 5 details |
|---|
| Sequence | GCCGTTTGCCCTCTTGTTTT | CACTTGCCCTGCTACGATGA | Start: 1163 |
| Tm | 59.896 | 60.108 | End: 1330 |
| Size | 20 | 20 | 168 |
(A)14: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GAAAACGTGCACCATGGCAT | ACCGACGTCTTCTGGAGAGA | Start: 299 |
| Tm | 60.038 | 59.964 | End: 402 |
| Size | 20 | 20 | 104 |
| Primer 2 details |
|---|
| Sequence | GCCGTTTGCCCTCTTGTTTT | TACAGCACTTGCCCTGCTAC | Start: 1163 |
| Tm | 59.896 | 60.036 | End: 1335 |
| Size | 20 | 20 | 173 |
| Primer 3 details |
|---|
| Sequence | TACCGCGAGTTTGGCATCAT | ATGCCATGGTGCACGTTTTC | Start: 107 |
| Tm | 60.108 | 60.038 | End: 318 |
| Size | 20 | 20 | 212 |
| Primer 4 details |
|---|
| Sequence | CCGCGAGTTTGGCATCATTT | ATGCCATGGTGCACGTTTTC | Start: 109 |
| Tm | 59.829 | 60.038 | End: 318 |
| Size | 20 | 20 | 210 |
| Primer 5 details |
|---|
| Sequence | GCCGTTTGCCCTCTTGTTTT | CACTTGCCCTGCTACGATGA | Start: 1163 |
| Tm | 59.896 | 60.108 | End: 1330 |
| Size | 20 | 20 | 168 |
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