ssr_primer
Primer Details for TRINITY_DN69833_c2_g1_i4 |
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| (A)14(ACAAACAAACAAACAAACAAAAAAAAAACGATTATATCGTCATTAATTATATTCGATATAATTATATTAGCTTTATTCATATAATTCGATTATATTATTGAAATAATATCGATTTACTCTTATTGTTAGCATTAAGAGGCAACGTAACAAAAATGAAGAATATCAACGATGATGTCACCACTGATAGCGTCTACGGCTAACTAAAGTTTTCTGTGCAGAGATCCAAAGTTTATAGTTTTGGAAAAGAAAGTCACCTCAGATGACCGAAATTTTTGGAGAAAAATACGGGTCGTAAGGCTATTAAGAACTTCGACTTCCAACATTCAAGGTCAGTTTCCATGCCTGAAAACTTCAGTTACCCGGCTTAATTTTCCAAATGAACCGAAATATTTCTTAACGTGCATTACAGCACTTCAAAGTCGACCCTGTTTAGTAATCCGGTGGGCTGGTCATTGC)(AAAC)4(A)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCTTTCACACGGGGGAAAAC | GTGCATGTTCCCTTGCCAAA | Start: 1371 |
| Tm | 59.968 | 59.608 | End: 1544 |
| Size | 20 | 20 | 174 |
| Primer 2 details |
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| Sequence | GTAGCTTTCACACGGGGGAA | GTGCATGTTCCCTTGCCAAA | Start: 1368 |
| Tm | 59.964 | 59.608 | End: 1544 |
| Size | 20 | 20 | 177 |
| Primer 3 details |
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| Sequence | GCTTTCACACGGGGGAAAAC | TAGTGCATGTTCCCTTGCCA | Start: 1371 |
| Tm | 59.968 | 59.596 | End: 1546 |
| Size | 20 | 20 | 176 |
| Primer 4 details |
|---|
| Sequence | GTAGCTTTCACACGGGGGAA | TAGTGCATGTTCCCTTGCCA | Start: 1368 |
| Tm | 59.964 | 59.596 | End: 1546 |
| Size | 20 | 20 | 179 |
| Primer 5 details |
|---|
| Sequence | AGCTTTCACACGGGGGAAAA | GTGCATGTTCCCTTGCCAAA | Start: 1370 |
| Tm | 60.106 | 59.608 | End: 1544 |
| Size | 20 | 20 | 175 |
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