ssr_primer
Primer Details for TRINITY_DN69825_c3_g2_i3 |
|---|
| (A)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTGCCATGTGTTACACCCGA | CTCTGCAGGAGGTTGTGAGG | Start: 2049 |
| Tm | 59.891 | 60.037 | End: 2306 |
| Size | 20 | 20 | 258 |
| Primer 2 details |
|---|
| Sequence | TTGCCCCGAATGTCAAGTCA | CTCTGCAGGAGGTTGTGAGG | Start: 2125 |
| Tm | 59.89 | 60.037 | End: 2306 |
| Size | 20 | 20 | 182 |
| Primer 3 details |
|---|
| Sequence | TTGCCCCGAATGTCAAGTCA | TTTTCACAGGAGTGCCAGCA | Start: 2125 |
| Tm | 59.89 | 60.107 | End: 2364 |
| Size | 20 | 20 | 240 |
| Primer 4 details |
|---|
| Sequence | GGGAAGCCAGAGGGTGAAAA | TGACTTGACATTCGGGGCAA | Start: 2004 |
| Tm | 59.888 | 59.89 | End: 2144 |
| Size | 20 | 20 | 141 |
| Primer 5 details |
|---|
| Sequence | GCCATGTGTTACACCCGACT | CTCTGCAGGAGGTTGTGAGG | Start: 2051 |
| Tm | 60.321 | 60.037 | End: 2306 |
| Size | 20 | 20 | 256 |
(A)36: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTGCCATGTGTTACACCCGA | CTCTGCAGGAGGTTGTGAGG | Start: 2049 |
| Tm | 59.891 | 60.037 | End: 2306 |
| Size | 20 | 20 | 258 |
| Primer 2 details |
|---|
| Sequence | TTGCCCCGAATGTCAAGTCA | CTCTGCAGGAGGTTGTGAGG | Start: 2125 |
| Tm | 59.89 | 60.037 | End: 2306 |
| Size | 20 | 20 | 182 |
| Primer 3 details |
|---|
| Sequence | TTGCCCCGAATGTCAAGTCA | TTTTCACAGGAGTGCCAGCA | Start: 2125 |
| Tm | 59.89 | 60.107 | End: 2364 |
| Size | 20 | 20 | 240 |
| Primer 4 details |
|---|
| Sequence | GGGAAGCCAGAGGGTGAAAA | TGACTTGACATTCGGGGCAA | Start: 2004 |
| Tm | 59.888 | 59.89 | End: 2144 |
| Size | 20 | 20 | 141 |
| Primer 5 details |
|---|
| Sequence | GCCATGTGTTACACCCGACT | CTCTGCAGGAGGTTGTGAGG | Start: 2051 |
| Tm | 60.321 | 60.037 | End: 2306 |
| Size | 20 | 20 | 256 |
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