ssr_primer
Primer Details for TRINITY_DN69813_c1_g1_i8 |
|---|
| (A)24: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TATGTGACGTCGCAAACCGA | TCGTTGAAGATTTGCCGGGA | Start: 322 |
| Tm | 60.039 | 59.964 | End: 501 |
| Size | 20 | 20 | 180 |
| Primer 2 details |
|---|
| Sequence | TCCCGGCAAATCTTCAACGA | TCATATCCCTCCCGCTCGAT | Start: 482 |
| Tm | 59.964 | 59.96 | End: 674 |
| Size | 20 | 20 | 193 |
| Primer 3 details |
|---|
| Sequence | TCCCGGCAAATCTTCAACGA | ATCATATCCCTCCCGCTCGA | Start: 482 |
| Tm | 59.964 | 59.96 | End: 675 |
| Size | 20 | 20 | 194 |
| Primer 4 details |
|---|
| Sequence | GTATGTGACGTCGCAAACCG | TCGTTGAAGATTTGCCGGGA | Start: 321 |
| Tm | 59.908 | 59.964 | End: 501 |
| Size | 20 | 20 | 181 |
| Primer 5 details |
|---|
| Sequence | TTGCCGACTTACACCGTTGA | TCGTTGAAGATTTGCCGGGA | Start: 351 |
| Tm | 59.896 | 59.964 | End: 501 |
| Size | 20 | 20 | 151 |
(A)12: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TATGTGACGTCGCAAACCGA | TCGTTGAAGATTTGCCGGGA | Start: 322 |
| Tm | 60.039 | 59.964 | End: 501 |
| Size | 20 | 20 | 180 |
| Primer 2 details |
|---|
| Sequence | TCCCGGCAAATCTTCAACGA | TCATATCCCTCCCGCTCGAT | Start: 482 |
| Tm | 59.964 | 59.96 | End: 674 |
| Size | 20 | 20 | 193 |
| Primer 3 details |
|---|
| Sequence | TCCCGGCAAATCTTCAACGA | ATCATATCCCTCCCGCTCGA | Start: 482 |
| Tm | 59.964 | 59.96 | End: 675 |
| Size | 20 | 20 | 194 |
| Primer 4 details |
|---|
| Sequence | GTATGTGACGTCGCAAACCG | TCGTTGAAGATTTGCCGGGA | Start: 321 |
| Tm | 59.908 | 59.964 | End: 501 |
| Size | 20 | 20 | 181 |
| Primer 5 details |
|---|
| Sequence | TTGCCGACTTACACCGTTGA | TCGTTGAAGATTTGCCGGGA | Start: 351 |
| Tm | 59.896 | 59.964 | End: 501 |
| Size | 20 | 20 | 151 |
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