ssr_primer
Primer Details for TRINITY_DN69806_c0_g2_i3 |
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| (A)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGGGTGCAAAATTAAGCGCA | CAGTGGTTGCGAAGTGAACG | Start: 3361 |
| Tm | 60.038 | 60.042 | End: 3549 |
| Size | 20 | 20 | 189 |
| Primer 2 details |
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| Sequence | GGAGATGGAGGGGTGCAAAA | CAGTGGTTGCGAAGTGAACG | Start: 3352 |
| Tm | 59.96 | 60.042 | End: 3549 |
| Size | 20 | 20 | 198 |
| Primer 3 details |
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| Sequence | GTCTCTCCTGACCAAGGCAC | CTCTATGACAGCCGGGCAAA | Start: 1390 |
| Tm | 60.037 | 60.108 | End: 1595 |
| Size | 20 | 20 | 206 |
| Primer 4 details |
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| Sequence | TTTGCCCGGCTGTCATAGAG | GCTCTCTGAAGGTGGCCTTT | Start: 1576 |
| Tm | 60.108 | 59.963 | End: 1689 |
| Size | 20 | 20 | 114 |
| Primer 5 details |
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| Sequence | TACATTGTCAAAGGCCGCCT | CTCTATGACAGCCGGGCAAA | Start: 1341 |
| Tm | 59.962 | 60.108 | End: 1595 |
| Size | 20 | 20 | 255 |
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