Primer Details for TRINITY_DN69801_c5_g1_i1 | |||
|---|---|---|---|
| (T)10ctttctttttaaccaatttaatcatcatccatccctaatagta(T)11: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGGAAAAAGCCATGGGAGCT | ACGCTGATTCTGACTTTTAAGAGT | Start: 19 |
| Tm | 59.589 | 58.457 | End: 286 |
| Size | 20 | 24 | 268 |
| Primer 2 details | |||
| Sequence | AGGAAAAAGCCATGGGAGCT | ACGCTGATTCTGACTTTTAAGAGTT | Start: 19 |
| Tm | 59.589 | 59.008 | End: 286 |
| Size | 20 | 25 | 268 |
| Primer 3 details | |||
| Sequence | ATGGGAGCTTGGCACCATC | ACGCTGATTCTGACTTTTAAGAGT | Start: 30 |
| Tm | 60.076 | 58.457 | End: 286 |
| Size | 19 | 24 | 257 |
| Primer 4 details | |||
| Sequence | AAGGAAAAAGCCATGGGAGCT | ACGCTGATTCTGACTTTTAAGAGT | Start: 18 |
| Tm | 60.203 | 58.457 | End: 286 |
| Size | 21 | 24 | 269 |
| Primer 5 details | |||
| Sequence | AAGGAAAAAGCCATGGGAGC | ACGCTGATTCTGACTTTTAAGAGT | Start: 18 |
| Tm | 58.73 | 58.457 | End: 286 |
| Size | 20 | 24 | 269 |
