ssr_primer
Primer Details for TRINITY_DN69782_c4_g4_i4 |
|---|
| (T)24: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACACGCGGTGCTTTTCTCTA | ACCTCATTGGCGGCTTCATA | Start: 58 |
| Tm | 59.967 | 59.454 | End: 263 |
| Size | 20 | 20 | 206 |
| Primer 2 details |
|---|
| Sequence | TGATGGATGTCCGTGTTGCA | ACCTCATTGGCGGCTTCATA | Start: 2 |
| Tm | 59.964 | 59.454 | End: 263 |
| Size | 20 | 20 | 262 |
| Primer 3 details |
|---|
| Sequence | GCGCTTTGCATTCCTCACTT | CCAGTGAAGCGGGCAAATTG | Start: 924 |
| Tm | 59.758 | 60.389 | End: 1063 |
| Size | 20 | 20 | 140 |
| Primer 4 details |
|---|
| Sequence | GATGTCCGTGTTGCATCTGC | ACCTCATTGGCGGCTTCATA | Start: 7 |
| Tm | 59.902 | 59.454 | End: 263 |
| Size | 20 | 20 | 257 |
| Primer 5 details |
|---|
| Sequence | TATGAAGCCGCCAATGAGGT | CGGCACATACGAGCTTCAGA | Start: 244 |
| Tm | 59.454 | 60.179 | End: 424 |
| Size | 20 | 20 | 181 |
(A)15caac(A)11: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACACGCGGTGCTTTTCTCTA | ACCTCATTGGCGGCTTCATA | Start: 58 |
| Tm | 59.967 | 59.454 | End: 263 |
| Size | 20 | 20 | 206 |
| Primer 2 details |
|---|
| Sequence | TGATGGATGTCCGTGTTGCA | ACCTCATTGGCGGCTTCATA | Start: 2 |
| Tm | 59.964 | 59.454 | End: 263 |
| Size | 20 | 20 | 262 |
| Primer 3 details |
|---|
| Sequence | GCGCTTTGCATTCCTCACTT | CCAGTGAAGCGGGCAAATTG | Start: 924 |
| Tm | 59.758 | 60.389 | End: 1063 |
| Size | 20 | 20 | 140 |
| Primer 4 details |
|---|
| Sequence | GATGTCCGTGTTGCATCTGC | ACCTCATTGGCGGCTTCATA | Start: 7 |
| Tm | 59.902 | 59.454 | End: 263 |
| Size | 20 | 20 | 257 |
| Primer 5 details |
|---|
| Sequence | TATGAAGCCGCCAATGAGGT | CGGCACATACGAGCTTCAGA | Start: 244 |
| Tm | 59.454 | 60.179 | End: 424 |
| Size | 20 | 20 | 181 |
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