Primer Details for TRINITY_DN69780_c10_g1_i1 | |||
|---|---|---|---|
| (A)12gaat(A)11gcaagaaaagagaaggaaaaaagaaagcaaagacaaaaaagagacagaaaaaaagataaaggaagagagagagaaaggtggagaataaaaaaagaag(A)10: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGGAAAGACACAAAGAAAAGAACA | TCCACCTTTCTCTCTCTCTTCCT | Start: 5 |
| Tm | 57.166 | 59.923 | End: 191 |
| Size | 24 | 23 | 187 |
| Primer 2 details | |||
| Sequence | AGGAAAGACACAAAGAAAAGAACA | TCCACCTTTCTCTCTCTCTTCC | Start: 5 |
| Tm | 57.166 | 58.564 | End: 191 |
| Size | 24 | 22 | 187 |
| Primer 3 details | |||
| Sequence | AGGAAAGACACAAAGAAAAGAACA | CCACCTTTCTCTCTCTCTTCCT | Start: 5 |
| Tm | 57.166 | 58.56 | End: 190 |
| Size | 24 | 22 | 186 |
| Primer 4 details | |||
| Sequence | AGGAAAGACACAAAGAAAAGAACA | CTCCACCTTTCTCTCTCTCTTCC | Start: 5 |
| Tm | 57.166 | 59.553 | End: 192 |
| Size | 24 | 23 | 188 |
| Primer 5 details | |||
| Sequence | AGGAAAGACACAAAGAAAAGAACAA | TCCACCTTTCTCTCTCTCTTCCT | Start: 5 |
| Tm | 57.773 | 59.923 | End: 191 |
| Size | 25 | 23 | 187 |
