ssr_primer
Primer Details for TRINITY_DN69764_c0_g1_i2 |
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| (T)32: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTGGAGAGTGTGACACAGGC | TCAAACACTGAGGGGGAAGG | Start: 54 |
| Tm | 59.893 | 59.229 | End: 156 |
| Size | 20 | 20 | 103 |
| Primer 2 details |
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| Sequence | TTGGAGAGTGTGACACAGGC | CAAACACTGAGGGGGAAGGC | Start: 54 |
| Tm | 59.893 | 60.896 | End: 155 |
| Size | 20 | 20 | 102 |
| Primer 3 details |
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| Sequence | TTGGAGAGTGTGACACAGGC | AAACACTGAGGGGGAAGGCT | Start: 54 |
| Tm | 59.893 | 61.068 | End: 154 |
| Size | 20 | 20 | 101 |
| Primer 4 details |
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| Sequence | TGGCTTGGACCAATTACAGCA | CTGAGGGGGAAGGCTGAAAG | Start: 6 |
| Tm | 60.203 | 60.035 | End: 149 |
| Size | 21 | 20 | 144 |
| Primer 5 details |
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| Sequence | TTGGAGAGTGTGACACAGGC | TTCAAACACTGAGGGGGAAGG | Start: 54 |
| Tm | 59.893 | 59.855 | End: 157 |
| Size | 20 | 21 | 104 |
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