ssr_primer
Primer Details for TRINITY_DN69734_c11_g2_i11 |
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| (T)18: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGTCACTGGGTGCAAATCCA | ACTTCTCCACCTAGGACGCT | Start: 688 |
| Tm | 59.815 | 59.96 | End: 805 |
| Size | 20 | 20 | 118 |
| Primer 2 details |
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| Sequence | TGGGTGCAGCTATTACACGG | TGGATTTGCACCCAGTGACA | Start: 514 |
| Tm | 60.108 | 59.815 | End: 707 |
| Size | 20 | 20 | 194 |
| Primer 3 details |
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| Sequence | CACTGGGCGTCAAGTTTTGG | GTTTGTCGCCATGTGCAGAA | Start: 1301 |
| Tm | 59.969 | 59.689 | End: 1506 |
| Size | 20 | 20 | 206 |
| Primer 4 details |
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| Sequence | CCACTGGGCGTCAAGTTTTG | GTTTGTCGCCATGTGCAGAA | Start: 1300 |
| Tm | 59.969 | 59.689 | End: 1506 |
| Size | 20 | 20 | 207 |
| Primer 5 details |
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| Sequence | TTAGGGGAGTGCAGCATGTG | GTTTGTCGCCATGTGCAGAA | Start: 1275 |
| Tm | 60.035 | 59.689 | End: 1506 |
| Size | 20 | 20 | 232 |
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