ssr_primer
Primer Details for TRINITY_DN69733_c3_g1_i3 |
|---|
| (T)15: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GACACACCCAGGTACAGTGG | CCCTCCCCTCAATACCTGGA | Start: 296 |
| Tm | 59.965 | 60.03 | End: 568 |
| Size | 20 | 20 | 273 |
| Primer 2 details |
|---|
| Sequence | GGACACACCCAGGTACAGTG | CCCTCCCCTCAATACCTGGA | Start: 295 |
| Tm | 59.965 | 60.03 | End: 568 |
| Size | 20 | 20 | 274 |
| Primer 3 details |
|---|
| Sequence | GTCCAGTTGTACGCATGGGA | CACTGTACCTGGGTGTGTCC | Start: 151 |
| Tm | 60.037 | 59.965 | End: 314 |
| Size | 20 | 20 | 164 |
| Primer 4 details |
|---|
| Sequence | GTCCAGTTGTACGCATGGGA | CCACTGTACCTGGGTGTGTC | Start: 151 |
| Tm | 60.037 | 59.965 | End: 315 |
| Size | 20 | 20 | 165 |
| Primer 5 details |
|---|
| Sequence | GACACACCCAGGTACAGTGG | CCTGGACAGGGTTGCAGAAT | Start: 296 |
| Tm | 59.965 | 59.961 | End: 554 |
| Size | 20 | 20 | 259 |
(T)18: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GACACACCCAGGTACAGTGG | CCCTCCCCTCAATACCTGGA | Start: 296 |
| Tm | 59.965 | 60.03 | End: 568 |
| Size | 20 | 20 | 273 |
| Primer 2 details |
|---|
| Sequence | GGACACACCCAGGTACAGTG | CCCTCCCCTCAATACCTGGA | Start: 295 |
| Tm | 59.965 | 60.03 | End: 568 |
| Size | 20 | 20 | 274 |
| Primer 3 details |
|---|
| Sequence | GTCCAGTTGTACGCATGGGA | CACTGTACCTGGGTGTGTCC | Start: 151 |
| Tm | 60.037 | 59.965 | End: 314 |
| Size | 20 | 20 | 164 |
| Primer 4 details |
|---|
| Sequence | GTCCAGTTGTACGCATGGGA | CCACTGTACCTGGGTGTGTC | Start: 151 |
| Tm | 60.037 | 59.965 | End: 315 |
| Size | 20 | 20 | 165 |
| Primer 5 details |
|---|
| Sequence | GACACACCCAGGTACAGTGG | CCTGGACAGGGTTGCAGAAT | Start: 296 |
| Tm | 59.965 | 59.961 | End: 554 |
| Size | 20 | 20 | 259 |
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