ssr_primer
Primer Details for TRINITY_DN69675_c0_g1_i6 |
|---|
| (A)14: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CTCAACTGGTCAGCCGTGAT | GGCCATACAATGAGAGCGGA | Start: 299 |
| Tm | 60.037 | 59.893 | End: 519 |
| Size | 20 | 20 | 221 |
| Primer 2 details |
|---|
| Sequence | ATCTTGGCTGGAAGGTGACG | TCACAACATTCAGGCGGGAA | Start: 2722 |
| Tm | 60.036 | 59.89 | End: 2835 |
| Size | 20 | 20 | 114 |
| Primer 3 details |
|---|
| Sequence | ATCTTGGCTGGAAGGTGACG | CATTCAGGCGGGAAAAGCTG | Start: 2722 |
| Tm | 60.036 | 59.828 | End: 2829 |
| Size | 20 | 20 | 108 |
| Primer 4 details |
|---|
| Sequence | GCTAGCCCGACTGACAATGA | ATCACGGCTGACCAGTTGAG | Start: 99 |
| Tm | 59.824 | 60.037 | End: 318 |
| Size | 20 | 20 | 220 |
| Primer 5 details |
|---|
| Sequence | AGCTAGCCCGACTGACAATG | ATCACGGCTGACCAGTTGAG | Start: 98 |
| Tm | 59.823 | 60.037 | End: 318 |
| Size | 20 | 20 | 221 |
(A)17: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CTCAACTGGTCAGCCGTGAT | GGCCATACAATGAGAGCGGA | Start: 299 |
| Tm | 60.037 | 59.893 | End: 519 |
| Size | 20 | 20 | 221 |
| Primer 2 details |
|---|
| Sequence | ATCTTGGCTGGAAGGTGACG | TCACAACATTCAGGCGGGAA | Start: 2722 |
| Tm | 60.036 | 59.89 | End: 2835 |
| Size | 20 | 20 | 114 |
| Primer 3 details |
|---|
| Sequence | ATCTTGGCTGGAAGGTGACG | CATTCAGGCGGGAAAAGCTG | Start: 2722 |
| Tm | 60.036 | 59.828 | End: 2829 |
| Size | 20 | 20 | 108 |
| Primer 4 details |
|---|
| Sequence | GCTAGCCCGACTGACAATGA | ATCACGGCTGACCAGTTGAG | Start: 99 |
| Tm | 59.824 | 60.037 | End: 318 |
| Size | 20 | 20 | 220 |
| Primer 5 details |
|---|
| Sequence | AGCTAGCCCGACTGACAATG | ATCACGGCTGACCAGTTGAG | Start: 98 |
| Tm | 59.823 | 60.037 | End: 318 |
| Size | 20 | 20 | 221 |
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