ssr_primer
Primer Details for TRINITY_DN69605_c3_g4_i5 |
|---|
| (T)23: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CTACGCGAGATTGGTACCCC | CGCAAGTATCGCACCGAAAG | Start: 635 |
| Tm | 59.968 | 59.975 | End: 879 |
| Size | 20 | 20 | 245 |
| Primer 2 details |
|---|
| Sequence | CCTACGCGAGATTGGTACCC | CGCAAGTATCGCACCGAAAG | Start: 634 |
| Tm | 59.968 | 59.975 | End: 879 |
| Size | 20 | 20 | 246 |
| Primer 3 details |
|---|
| Sequence | ACCCCGTTACGACTGTTCAC | CGCAAGTATCGCACCGAAAG | Start: 650 |
| Tm | 59.967 | 59.975 | End: 879 |
| Size | 20 | 20 | 230 |
| Primer 4 details |
|---|
| Sequence | GCGACTTCGTCAATTCTCGC | ATAGCACCTGAGCGCTTGTT | Start: 1428 |
| Tm | 59.976 | 60.036 | End: 1601 |
| Size | 20 | 20 | 174 |
| Primer 5 details |
|---|
| Sequence | GCGACTTCGTCAATTCTCGC | AACATAGCACCTGAGCGCTT | Start: 1428 |
| Tm | 59.976 | 60.036 | End: 1604 |
| Size | 20 | 20 | 177 |
(T)11atttttttta(T)13attttatacctctgagaaaattgtcttttcaaaaaaaacgattacatcaattggtgg(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CTACGCGAGATTGGTACCCC | CGCAAGTATCGCACCGAAAG | Start: 635 |
| Tm | 59.968 | 59.975 | End: 879 |
| Size | 20 | 20 | 245 |
| Primer 2 details |
|---|
| Sequence | CCTACGCGAGATTGGTACCC | CGCAAGTATCGCACCGAAAG | Start: 634 |
| Tm | 59.968 | 59.975 | End: 879 |
| Size | 20 | 20 | 246 |
| Primer 3 details |
|---|
| Sequence | ACCCCGTTACGACTGTTCAC | CGCAAGTATCGCACCGAAAG | Start: 650 |
| Tm | 59.967 | 59.975 | End: 879 |
| Size | 20 | 20 | 230 |
| Primer 4 details |
|---|
| Sequence | GCGACTTCGTCAATTCTCGC | ATAGCACCTGAGCGCTTGTT | Start: 1428 |
| Tm | 59.976 | 60.036 | End: 1601 |
| Size | 20 | 20 | 174 |
| Primer 5 details |
|---|
| Sequence | GCGACTTCGTCAATTCTCGC | AACATAGCACCTGAGCGCTT | Start: 1428 |
| Tm | 59.976 | 60.036 | End: 1604 |
| Size | 20 | 20 | 177 |
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