ssr_primer
Primer Details for TRINITY_DN69580_c3_g3_i3 |
|---|
| (TCA)6: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GACACGACAAGACCTAGCCC | CTTTATCTTCTGCGGGCCGA | Start: 1726 |
| Tm | 60.109 | 60.179 | End: 1827 |
| Size | 20 | 20 | 102 |
| Primer 2 details |
|---|
| Sequence | CCCGACACGACAAGACCTAG | CTTTATCTTCTGCGGGCCGA | Start: 1723 |
| Tm | 59.829 | 60.179 | End: 1827 |
| Size | 20 | 20 | 105 |
| Primer 3 details |
|---|
| Sequence | CGACCAAAGCAATCGCGTAG | AAGATGGCTCTTGCTGCTGA | Start: 1416 |
| Tm | 59.975 | 59.673 | End: 1587 |
| Size | 20 | 20 | 172 |
| Primer 4 details |
|---|
| Sequence | AACACGACCAAAGCAATCGC | AAGATGGCTCTTGCTGCTGA | Start: 1412 |
| Tm | 60.04 | 59.673 | End: 1587 |
| Size | 20 | 20 | 176 |
| Primer 5 details |
|---|
| Sequence | AGGGCAGGCCTAATACCACT | TGGTCGTACCACCAAACAGG | Start: 663 |
| Tm | 60.328 | 59.893 | End: 821 |
| Size | 20 | 20 | 159 |
(T)17: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GACACGACAAGACCTAGCCC | CTTTATCTTCTGCGGGCCGA | Start: 1726 |
| Tm | 60.109 | 60.179 | End: 1827 |
| Size | 20 | 20 | 102 |
| Primer 2 details |
|---|
| Sequence | CCCGACACGACAAGACCTAG | CTTTATCTTCTGCGGGCCGA | Start: 1723 |
| Tm | 59.829 | 60.179 | End: 1827 |
| Size | 20 | 20 | 105 |
| Primer 3 details |
|---|
| Sequence | CGACCAAAGCAATCGCGTAG | AAGATGGCTCTTGCTGCTGA | Start: 1416 |
| Tm | 59.975 | 59.673 | End: 1587 |
| Size | 20 | 20 | 172 |
| Primer 4 details |
|---|
| Sequence | AACACGACCAAAGCAATCGC | AAGATGGCTCTTGCTGCTGA | Start: 1412 |
| Tm | 60.04 | 59.673 | End: 1587 |
| Size | 20 | 20 | 176 |
| Primer 5 details |
|---|
| Sequence | AGGGCAGGCCTAATACCACT | TGGTCGTACCACCAAACAGG | Start: 663 |
| Tm | 60.328 | 59.893 | End: 821 |
| Size | 20 | 20 | 159 |
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