ssr_primer
Primer Details for TRINITY_DN69575_c4_g1_i5 |
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| (A)12taaccggcgaccacacccaagataatcgccgcggatcacttagatcggacttaaatttgtgt(C)12: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ATAATCGCCGCGGATCACTT | GATGCTGAGAGGGCGATTCA | Start: 94 |
| Tm | 59.966 | 59.894 | End: 239 |
| Size | 20 | 20 | 146 |
| Primer 2 details |
|---|
| Sequence | GGCGACCACACCCAAGATAA | GATGCTGAGAGGGCGATTCA | Start: 78 |
| Tm | 60.036 | 59.894 | End: 239 |
| Size | 20 | 20 | 162 |
| Primer 3 details |
|---|
| Sequence | ATAATCGCCGCGGATCACTT | CTGAGAGGGCGATTCACGTT | Start: 94 |
| Tm | 59.966 | 60.109 | End: 235 |
| Size | 20 | 20 | 142 |
| Primer 4 details |
|---|
| Sequence | GGCGACCACACCCAAGATAA | CTGAGAGGGCGATTCACGTT | Start: 78 |
| Tm | 60.036 | 60.109 | End: 235 |
| Size | 20 | 20 | 158 |
| Primer 5 details |
|---|
| Sequence | ATAATCGCCGCGGATCACTT | ATGCTGAGAGGGCGATTCAC | Start: 94 |
| Tm | 59.966 | 60.179 | End: 238 |
| Size | 20 | 20 | 145 |
(A)28: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ATAATCGCCGCGGATCACTT | GATGCTGAGAGGGCGATTCA | Start: 94 |
| Tm | 59.966 | 59.894 | End: 239 |
| Size | 20 | 20 | 146 |
| Primer 2 details |
|---|
| Sequence | GGCGACCACACCCAAGATAA | GATGCTGAGAGGGCGATTCA | Start: 78 |
| Tm | 60.036 | 59.894 | End: 239 |
| Size | 20 | 20 | 162 |
| Primer 3 details |
|---|
| Sequence | ATAATCGCCGCGGATCACTT | CTGAGAGGGCGATTCACGTT | Start: 94 |
| Tm | 59.966 | 60.109 | End: 235 |
| Size | 20 | 20 | 142 |
| Primer 4 details |
|---|
| Sequence | GGCGACCACACCCAAGATAA | CTGAGAGGGCGATTCACGTT | Start: 78 |
| Tm | 60.036 | 60.109 | End: 235 |
| Size | 20 | 20 | 158 |
| Primer 5 details |
|---|
| Sequence | ATAATCGCCGCGGATCACTT | ATGCTGAGAGGGCGATTCAC | Start: 94 |
| Tm | 59.966 | 60.179 | End: 238 |
| Size | 20 | 20 | 145 |
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